在被猴病毒40转化的人类细胞表面出现较小的甘露糖基糖肽。
Appearance of smaller mannosyl-glycopeptides on the surface of a human cell transformed by simian virus 40.
作者信息
Ceccarini C
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2687-90. doi: 10.1073/pnas.72.7.2687.
When fucosyl surface glycopeptides from growing normal human cells (WI 38) were compared with those derived from nongrowing cells the former were enriched in high-molecular-weight species. However, a line of human cells (WI 18Va) transformed by simian virus 40 appeared to have fucosyl-glycopeptides similar in size distribution to those from rapidly growing non-transformed cells (WI 38). I propose that the enrichment in high molecular weight species in these cells might be growth- rather than transformation-dependent. Using radioactive mannose to label surface glycopeptides, I observed that those derived from transformed cells (WI 18Va) were smaller than those from rapidly growing normal cells. Thus differences in size distribution may not be adequate criteria to evaluate the growth-dependent alterations in cell surface glycopeptides.
将来自正在生长的正常人细胞（WI 38）的岩藻糖基化表面糖肽与来自非生长细胞的糖肽进行比较时，前者富含高分子量物质。然而，由猿猴病毒40转化的人细胞系（WI 18Va）似乎具有与快速生长的未转化细胞（WI 38）大小分布相似的岩藻糖基化糖肽。我认为这些细胞中高分子量物质的富集可能是生长依赖性的，而非转化依赖性的。使用放射性甘露糖标记表面糖肽时，我观察到来自转化细胞（WI 18Va）的糖肽比来自快速生长的正常细胞的糖肽小。因此，大小分布的差异可能不是评估细胞表面糖肽生长依赖性改变的充分标准。
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