He Lan, Cai Yu, Chen Zhao-Hui, Jiang Jian-Min, Wang Ning-Sheng
Institute of Clinical Pharmacology, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Zhongguo Zhong Yao Za Zhi. 2006 Aug;31(16):1358-60.
To investigate the effect of traditional classical compound Wulingsan on renal hemodynamic in rats with adriamycin (ADR)-induced nephrosis.
After establishing a model of rats with adriamycin-induced nephrosis, we administrated wulin-san to the ADR rats via oral gavage for four weeks and measured mean arterial blood preasure (MABP) with manometer. Renal clearance of paraaminohippuric acid (PAH) and inulin were detected, then renal plasma flow (RPF) and glomerular filtration rate (GFR) were calculated. Renal vascular resistance (RVR) was calculated as the division of MABP by RPF. Renal endothelin (ET) and angiotensin II (Ang II) were detected with radioimmunity assay kits, and nitrous oxide (NO) was detected with biochemical kits.
There was no significant change of GFR in ARD rats, but RPF and NO were decreased, which accompanied by enhanced RVR, ET and Ang II. RPF was increased in the administrated rats, in company with RVR, ET and Ang II decreased, whereas NO was not influenced after the administration.
Wulingsan can improve the renal hemodynamic in ADR rats, at least in part by modulating the levels of vasoactive factor.
探讨传统经典复方五苓散对阿霉素（ADR）诱导的肾病大鼠肾血流动力学的影响。
建立阿霉素诱导的肾病大鼠模型后，通过灌胃给予五苓散4周，用压力计测量平均动脉血压（MABP）。检测对氨基马尿酸（PAH）和菊粉的肾清除率，然后计算肾血浆流量（RPF）和肾小球滤过率（GFR）。肾血管阻力（RVR）通过MABP除以RPF计算得出。用放射免疫分析试剂盒检测肾内皮素（ET）和血管紧张素II（Ang II），用生化试剂盒检测一氧化氮（NO）。
阿霉素诱导肾病大鼠的GFR无显著变化，但RPF和NO降低，同时RVR、ET和Ang II升高。给药大鼠的RPF升高，同时RVR、ET和Ang II降低，而给药后NO未受影响。
五苓散可改善阿霉素诱导肾病大鼠的肾血流动力学，至少部分是通过调节血管活性因子水平实现的。
