Moravec M, Moravec J, Forsgren S
Laboratoire d'Energétique et de Cardiologie Cellulaire, INSERM, Faculté de Pharmacie, Dijon, France.
Cell Tissue Res. 1990 Nov;262(2):315-27. doi: 10.1007/BF00309887.
Immunohistochemical properties of the terminal nerve network in the rat heart were assessed by use of the elution-restaining method. The colocalization of the enzymes involved in catecholamine synthesis (tyrosine hydroxylase--TH. dopamine-beta-hydroxylase--DBH) as well as the respective distributions of the neuropeptides associated with the adrenergic nervous system (neuropeptide tyrosine--NPY, C-terminal flanking peptide of neuropeptide Y--C-PON) were studied in series of serial sections throughout the interatrial septum and the atrioventricular junction. Our data suggest that ganglion cells of sulcus terminalis as well as the epicardial ganglia enclosed between the superior vena cava and ascending aorta are VIP- and TH-negative, but neuropeptide Y- and DBH-immunoreactive. They give rise to three intraseptal nerves directed towards the specialised structures of the atrioventricular junction. These nerve fascicles contain abundant, thick TH-immunoreactive nerve fibres and scarce, thin NPY- and DBH-immunoreactive fibres. The cell bodies of the intramural ganglion cells localized between the right and left branches of the bundle of His (Moravec and Moravec 1984) are strongly TH- and DBH-immunoreactive. They are innervated by thick nerve fibres having the same immunohistochemical properties (NPY- and DBH-immunoreactivities) as those of a subpopulation of the epicardial ganglion cells and seem to supply some of the TH-immunoreactive nerve fibres directed via the intraseptal nerves to the epicardial ganglia. The existence of a multicomponent nerve network, characterized by a reciprocal innervation of the sinus node and atrioventricular node areas, is suggested by our immunohistochemical data.
采用洗脱保留法评估大鼠心脏终末神经网络的免疫组织化学特性。在整个房间隔和房室交界处的系列连续切片中，研究了参与儿茶酚胺合成的酶（酪氨酸羟化酶 - TH、多巴胺 - β - 羟化酶 - DBH）的共定位以及与肾上腺素能神经系统相关的神经肽（神经肽Y - NPY、神经肽Y的C末端侧翼肽 - C - PON）的各自分布。我们的数据表明，终沟神经节细胞以及上腔静脉和升主动脉之间的心外膜神经节是VIP和TH阴性，但神经肽Y和DBH免疫反应阳性。它们发出三条间隔内神经，指向房室交界处的特殊结构。这些神经束含有丰富的、粗大的TH免疫反应性神经纤维以及稀少的、细小的NPY和DBH免疫反应性纤维。位于希氏束左右分支之间的壁内神经节细胞（Moravec和Moravec，1984）的细胞体强烈TH和DBH免疫反应阳性。它们由具有与心外膜神经节细胞亚群相同免疫组织化学特性（NPY和DBH免疫反应性）的粗大神经纤维支配，并且似乎为一些通过间隔内神经导向心外膜神经节的TH免疫反应性神经纤维提供支持。我们的免疫组织化学数据提示存在一个多成分神经网络，其特征为窦房结和房室结区域的相互支配。
