Hu Sui-yu, Zhang Chun-hu, Cao Mei-qun, Peng Gui-jun, Huang Chuan-yuan
Institute of Combination of Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2006 Oct;31(5):676-81.
To investigate the effect of Baisong tablets on the nerve-biochemistry and neuroendocrine of chronic mild unpredicted stress depression in rats.
Sixty male Sprague-Dawley rats were randomly allocated to 4 groups: a normal control (NC), a model control (MC), a fluoxetine control (FC) and a Baisong tablet treatment group (BST). All rats except the control group were singly housed and exposed to an unpredicted sequence of mild stressor. The levels of serotonin (5-HT), glutamate (Glu) and gama-aminobutyric acid (GABA) of the hippocampus were measured by high-performance liquid chromatography. The concentration of corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and cortisol (CORT) in the plasma of the rats were detected by radio-immunity. The CRHmRNA expressions in the hypothalamus and prefrontal cortex were semiquantified by RT-PCR method. The differences of BDNF and TrkB protein expression in CA1 and CA3 pyramidal and dentate gyrus granule cells layers of hippocampus were investigated with immunohistochemistry technique.
In comparison with NC group, the levels of 5-HT, and GABA of the hippocampus in MC rats reduced significantly (P<0.01), and the concentration of CRH, ACTH, and CORT of the plasma and the level of Glu of the hippocampus and CRH mRNA expression in the brain increased significantly. Fluoxetine or Baisong could significantly regulate the abnormal changes of all the above.
Chronic mild unpredicted stress can affect neuroendoerine and cause depression, and Baisong tablet has antagonism against it.
探讨百松片对慢性轻度不可预测应激抑郁大鼠神经生化及神经内分泌的影响。
将60只雄性Sprague-Dawley大鼠随机分为4组：正常对照组（NC）、模型对照组（MC）、氟西汀对照组（FC）和百松片治疗组（BST）。除对照组外，所有大鼠单笼饲养，并暴露于不可预测的轻度应激源序列中。采用高效液相色谱法测定海马中5-羟色胺（5-HT）、谷氨酸（Glu）和γ-氨基丁酸（GABA）的水平。采用放射免疫法检测大鼠血浆中促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）和皮质醇（CORT）的浓度。采用RT-PCR法对下丘脑和前额叶皮质中CRHmRNA的表达进行半定量分析。采用免疫组织化学技术研究海马CA1、CA3锥体细胞层和齿状回颗粒细胞层中脑源性神经营养因子（BDNF）和酪氨酸激酶受体B（TrkB）蛋白表达的差异。
与NC组相比，MC组大鼠海马中5-HT和GABA水平显著降低（P<0.01），血浆中CRH、ACTH和CORT浓度、海马中Glu水平及脑中CRHmRNA表达显著升高。氟西汀或百松均可显著调节上述所有异常变化。
慢性轻度不可预测应激可影响神经内分泌并导致抑郁，百松片对此具有拮抗作用。
