Li Yun-hui, Zhang Chun-hu, Wang Su-e, Qiu Juan, Hu Sui-yu, Xiao Gui-lin
Institute of Integrative Medicine, Key Unit of State Administration of Traditional Chinese Medicine of the Peoples's Republic of China, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.
Zhong Xi Yi Jie He Xue Bao. 2009 Nov;7(11):1073-7. doi: 10.3736/jcim20091110.
To investigate the effects of Chaihu Shugan San (CHSGS), a compound traditional Chinese herbal medicine, on behavior and plasma levels of corticotropin releasing hormone (CRH) and adrenocorticotropic hormone (ACTH) of rats with chronic mild unpredicted stress depression.
Forty male Sprague-Dawley rats were randomly divided into 4 groups: normal control group, untreated group, fluoxetine group and CHSGS group. Except the normal control group, rats were singly housed and exposed to an unpredicted sequence of mild stressor for continuous 4 weeks to induce depression. Since the fifteenth day, rats were intragastrically administered with equal volume agents respectively for 2 weeks [normal saline for the normal control group and the untreated group, fluoxetine (1.8 mg/kg) for the fluoxetine group and CHSGS (5.9 g/kg) for the CHSGS group]. Behavioral scores of rats were detected by open-field test and sucrose preference test, and the plasma levels of CRH and ACTH in different groups were detected by radioimmunoassay.
Compared with the normal control group, body weights of the rats in the untreated group were significantly decreased. Scores of crossing, rears and grooming in open-field test were reduced significantly. Pure water consumption in sucrose preference test was increased significantly. The levels of plasma CRH and ACTH were significantly increased. The depressive behaviors of the rats were improved significantly and the levels of plasma CRH and ACTH were obviously reduced in the CHSGS group.
Chronic mild unpredicted mild stress can affect the neuroendocrine and behavior and cause depression in rats. CHSGS can regulate HPA hyperactivity of rats caused by chronic stress and has antidepressive effects.
探讨中药复方柴胡疏肝散对慢性轻度不可预测应激抑郁模型大鼠行为及血浆促肾上腺皮质激素释放激素（CRH）和促肾上腺皮质激素（ACTH）水平的影响。
将40只雄性Sprague-Dawley大鼠随机分为4组：正常对照组、未治疗组、氟西汀组和柴胡疏肝散组。除正常对照组外，其余大鼠单笼饲养，连续4周接受不可预测的轻度应激刺激以诱导抑郁。自第15天起，分别给予各组大鼠等体积药物灌胃2周[正常对照组和未治疗组给予生理盐水，氟西汀组给予氟西汀（1.8 mg/kg），柴胡疏肝散组给予柴胡疏肝散（5.9 g/kg）]。采用旷场试验和蔗糖偏好试验检测大鼠行为学评分，放射免疫法检测各组大鼠血浆CRH和ACTH水平。
与正常对照组相比，未治疗组大鼠体重显著下降，旷场试验中穿越、直立和修饰得分显著降低，蔗糖偏好试验中纯净水消耗量显著增加，血浆CRH和ACTH水平显著升高。柴胡疏肝散组大鼠抑郁行为明显改善，血浆CRH和ACTH水平明显降低。
慢性轻度不可预测性轻度应激可影响大鼠神经内分泌及行为，导致大鼠抑郁。柴胡疏肝散可调节慢性应激所致大鼠HPA轴功能亢进，具有抗抑郁作用。
