Leandro Carol Góis, Martins de Lima Thais, Folador Alessandra, Alba-Loreiro Tatiana, do Nascimento Elizabeth, Manhães de Castro Raul, de Castro Célia M M B, Pithon-Curi Tânia, Curi Rui
Superior School of Physical Education, Federal University of Pernambuco, Recife, Brazil.
Neuroimmunomodulation. 2006;13(2):105-13. doi: 10.1159/000096432. Epub 2006 Oct 23.
BACKGROUND/AIMS: Modulations in the immune function by stress are a well-known phenomenon. Acute restraint stress may induce impaired T-lymphocyte responses. Moderate physical training is associated with beneficial effects on immunological functions. We investigated the effects of a moderate physical training on T-lymphocyte function in rats submitted to acute restraint stress.
Thirty male Wistar rats weighing 210-226 g were randomly divided into four groups: non-trained rats (NT, n = 7), and non-trained rats submitted to stress (NT + S, n = 8); trained rats (T, n = 7), and trained rats submitted to stress (T + S, n = 8). Trained rats were submitted to a program of moderate running over a period of 8 weeks. Rats subjected to restraint stress were kept immobilized in glass cylinders (8 cm in diameter and 24 cm long) during 60 min. Plasma corticosterone concentration, peripheral blood leukocyte number, indicators of apoptosis of T lymphocytes in blood and lymphoid organs, and mitogen-induced proliferation of T lymphocytes in lymphoid organs were evaluated.
Acute stress exposure raised plasma corticosterone concentration (p < 0.001), but not in previously trained animals. Restraint stress induced an increase in the percentage of lymphocytes in apoptosis, and a decrease in the concanavalin-A-induced proliferation of lymphocytes from the thymus and lymph nodes, and an increase in lymphocytes of the spleen. Neither of these alterations was observed in trained animals submitted to acute restraint stress.
Our data confirm that acute restraint stress is associated with changes in T-lymphocyte function. Moreover, moderate physical training attenuates the effects of acute stress by a mechanism that involves the hypothalamic-pituitary-adrenal axis and an increase in tolerance of leukocytes.
背景/目的：应激对免疫功能的调节是一种众所周知的现象。急性束缚应激可能会导致T淋巴细胞反应受损。适度的体育锻炼对免疫功能具有有益影响。我们研究了适度体育锻炼对遭受急性束缚应激的大鼠T淋巴细胞功能的影响。
将30只体重210 - 226 g的雄性Wistar大鼠随机分为四组：未训练大鼠（NT，n = 7）、遭受应激的未训练大鼠（NT + S，n = 8）、训练大鼠（T，n = 7）、遭受应激的训练大鼠（T + S，n = 8）。训练大鼠接受为期8周的适度跑步训练计划。遭受束缚应激的大鼠在玻璃圆筒（直径8 cm，长24 cm）中固定60分钟。评估血浆皮质酮浓度、外周血白细胞数量、血液和淋巴器官中T淋巴细胞凋亡指标以及淋巴器官中丝裂原诱导的T淋巴细胞增殖情况。
急性应激暴露使血浆皮质酮浓度升高（p < 0.001），但在先前训练过的动物中未出现这种情况。束缚应激导致凋亡淋巴细胞百分比增加，胸腺和淋巴结中伴刀豆球蛋白A诱导的淋巴细胞增殖减少，脾脏淋巴细胞增加。在遭受急性束缚应激的训练动物中未观察到这些改变。
我们的数据证实急性束缚应激与T淋巴细胞功能变化有关。此外，适度体育锻炼通过涉及下丘脑 - 垂体 - 肾上腺轴的机制和白细胞耐受性增加来减轻急性应激的影响。
