Robles Valderrama E, Hurtado Bocanegra M D, González Arreaga M E, Ramírez García P, Durán Díaz A, Ayala Patiño R, Martínez Pérez M E
Unidad de Investigación Interdisciplinaria en Ciencias de la Salud y la Educación, UNAM FES Iztacala, México.
Rev Latinoam Microbiol. 2002 Apr-Jun;44(2):69-74.
Shigella boydii, Shigella flexneri, Shigella dysenteriae and Shigella sonnei were identified using gas chromatography instead of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 192 samples were analyzed both reference strains (ATCC 8700, INDRE B2188, B2194 and B2199) and environmental isolates. 12 fatty acids were included in the profiles from which 3-hydroxytetradecanoic acid (peak 12), trans 9-octadecanoic acid (peak 22), heptadecanoic acid (peak 18) and octadecanoic acid (peak 23), were the most important for the differentiation of the species analyzed.
