Watanabe K
Department of Orthopaedic Surgery, hamamatsu, University School of Medicine, Shizuoka, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1990 Dec;64(12):1202-14.
Spontaneous non-obese diabetic (NOD) mice were used to study the morphological change of muscle fibers in diabetes mellitus. The gastrocnemius and soleus muscles were removed from the NOD mice suffering from diabetes mellitus for 1, 3, 6 or 9 weeks. Muscle fibers were observed electronmicroscopically and histochemically, by staining with Sudan black B. Single fiber atrophy was noted in white fibers at an early stage of the illness. At the age of 6 and 9 weeks, white fibers showed extensive damage, characterized by the atrophy and wide separation of myofibrils, irregularity and thinness of myofilaments and massive glycogen deposition. The damage in the myofibril itself, however, was less pronounced in red and intermediate fibers, and those fibers had more abnormal mitochondria and a decreased affinity for sudan pigment. Morphological changes were varied in the three types of fibers. It is concluded that diabetes mellitus had different effects on each three types of muscle fibers neurogenically and metabolically.
采用自发性非肥胖型糖尿病（NOD）小鼠研究糖尿病时肌纤维的形态变化。从患有糖尿病1、3、6或9周的NOD小鼠身上取出腓肠肌和比目鱼肌。通过用苏丹黑B染色，在电子显微镜下和组织化学上观察肌纤维。在疾病早期，白色纤维出现单纤维萎缩。在6周和9周龄时，白色纤维显示出广泛损伤，其特征为肌原纤维萎缩和广泛分离、肌丝不规则和变细以及大量糖原沉积。然而，肌原纤维本身的损伤在红色和中间纤维中不太明显，并且这些纤维有更多异常线粒体以及对苏丹色素的亲和力降低。三种类型的纤维形态变化各不相同。得出的结论是，糖尿病对三种类型的肌纤维在神经源性和代谢性方面有不同影响。
