Chou C T, Lee W, Chang M L, Lee C F, Kuo S Y, Lin Y M
Department of Medicine, Tri-Service General Hospital, Taipei, Taiwan, R.O.C.
J Formos Med Assoc. 1990 Oct;89(10):845-9.
A previous study demonstrated that interferon was present in the serum of 30% of the patients with systemic lupus erythematosus (SLE), which was significantly higher than the 4.5% found in normal controls. We also recently reported that interferon production was deficient from SLE mononuclear cells, which has been attributed to immunodeficiency of the lymphocytes. In this study, interferon measurement included lymphocytes obtained from peripheral blood (PB), synovial fluid (SF) and synovial tissue (ST) in patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS). PB from normal subjects (NS) was used as a control. The results showed with PHA stimulation, that the interferon level in PBL (L = lymphocyte) in NS (70.0 +/- 67.5) was significantly higher when compared with PBL in RA (27.9 +/- 21.6). However, there was no difference between PBL in NS and AS. With ConA stimulation, the interferon level was significantly higher in the PBL of NS (130 +/- 59) and as compared with the PBL in RA (83.6 +/- 53.5). The SFL in RA (67.8 +/- 31.1) and the STL in RA (77.2 +/- 93.2) were also significantly different. It is concluded that interferon production was deficient not only in PBL in RA, but also in SF and STL in RA. The reduced interferon production from PB, SF and ST lymphocytes in RA patients may be due to previous release or immunodeficiency. Lymphocyte interferon production was normal in AS, which suggests that the lymphocyte abnormality between RA and AS may be different.
先前的一项研究表明，30%的系统性红斑狼疮（SLE）患者血清中存在干扰素，这一比例显著高于正常对照组中的4.5%。我们最近还报告称，SLE单核细胞的干扰素产生存在缺陷，这归因于淋巴细胞的免疫缺陷。在本研究中，对类风湿关节炎（RA）和强直性脊柱炎（AS）患者外周血（PB）、滑液（SF）和滑膜组织（ST）中的淋巴细胞进行了干扰素检测。以正常受试者（NS）的PB作为对照。结果显示，经PHA刺激后，NS组外周血淋巴细胞（L = 淋巴细胞）中的干扰素水平（70.0±67.5）显著高于RA组外周血淋巴细胞中的水平（27.9±21.6）。然而，NS组和AS组外周血淋巴细胞之间无差异。经ConA刺激后，NS组外周血淋巴细胞中的干扰素水平（130±59）显著高于RA组外周血淋巴细胞中的水平（83.6±53.5）。RA组的滑液淋巴细胞（SFL，67.8±31.1）和RA组的滑膜组织淋巴细胞（STL，77.2±93.2）也存在显著差异。结论是，RA患者不仅外周血淋巴细胞中的干扰素产生存在缺陷，其滑液和滑膜组织淋巴细胞中也是如此。RA患者外周血、滑液和滑膜组织淋巴细胞产生的干扰素减少可能是由于先前的释放或免疫缺陷。AS患者淋巴细胞产生干扰素正常，这表明RA和AS患者的淋巴细胞异常情况可能不同。
