Conners Shannon B, Mongodin Emmanuel F, Johnson Matthew R, Montero Clemente I, Nelson Karen E, Kelly Robert M
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
FEMS Microbiol Rev. 2006 Nov;30(6):872-905. doi: 10.1111/j.1574-6976.2006.00039.x.
High-throughput sequencing of microbial genomes has allowed the application of functional genomics methods to species lacking well-developed genetic systems. For the model hyperthermophile Thermotoga maritima, microarrays have been used in comparative genomic hybridization studies to investigate diversity among Thermotoga species. Transcriptional data have assisted in prediction of pathways for carbohydrate utilization, iron-sulfur cluster synthesis and repair, expolysaccharide formation, and quorum sensing. Structural genomics efforts aimed at the T. maritima proteome have yielded hundreds of high-resolution datasets and predicted functions for uncharacterized proteins. The information gained from genomics studies will be particularly useful for developing new biotechnology applications for T. maritima enzymes.
微生物基因组的高通量测序使得功能基因组学方法能够应用于那些缺乏完善遗传系统的物种。对于嗜热栖热袍菌这种模式嗜热菌，微阵列已被用于比较基因组杂交研究，以探究栖热袍菌属物种间的多样性。转录数据有助于预测碳水化合物利用、铁硫簇合成与修复、胞外多糖形成以及群体感应的途径。针对嗜热栖热袍菌蛋白质组的结构基因组学研究已经产生了数百个高分辨率数据集，并预测了未表征蛋白质的功能。从基因组学研究中获得的信息对于开发嗜热栖热袍菌酶的新生物技术应用将特别有用。
