Zhao Qin, Shen Li-xin, Zhang Hong, Wu Xian-ping, Xie Hui, Shan Peng-fei, Cao Xing-zhi, Liao Er-yuan, Luo Xiang-hang
Institute of Endocrinology and Metabolism, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Zhonghua Yi Xue Za Zhi. 2006 Jul 25;86(28):1957-61.
To study the age-related changes of bone formation markers, i.e., serum bone alkaline phosphatase (sBAP) and serum osteocalcin (sOC), bone resorption marker, i.e., cross-linked N-telopeptides of type 1 collagen (sNTX), and bone mineral density (BMD) in healthy men.
Serum sBAP, sOC, and sNTX of 389 randomly selected males, aged 20 - 80, all of Han nationality, were measured by using enzyme-linked immunosorbent (ELISA). Dual energy X-ray densitometer was used to measure the BMD of the lumbar vertebrae, left femoral neck, Ward's triangle, and hip. The relationships of these markers to age were analyzed.
(1) The sBAP, sOC, and sNTX were negatively correlated with age, the cubic regression model being better with age-related changes of bone biochemical markers as compared with the other regression models (R(2) = 0.013 - 0.029, P < 0.05); (2) When all subjects were stratified by 10-years, the bone biochemical marker values were the highest in the age group 20 - 29, with the sBAP of 30.9 U/L +/- 12.6 U/L, sOC of 12.6 microg/L +/- 6.2 microg/L, and sNTX of 18.2 micromol/L +/- 6.6 micromol/L; then decreased with aging and to a nadir level in the age group 50 - 59, with the sBAP of 26.9 U/L +/- 8.6 U/L, sOC of 9.2 microg/L +/- 5.3 microg/L, and sNTX of 15.6 micromol/L +/- 6.1 micromol/L. After the age of 60, sNTX increased slightly 16.0 micromol/L +/- 6.1 micromol/L, however, BAP and sOC remained stable; (3) After adjustment for age, height, weight, BMI and smoking, serum BAP was negatively correlated with BMD of multiple skeletal sites. sOC was inversely associated with BMD of multiple skeletal sites except lateral spine; and sNTX was negatively correlated with BMD of the lumbar spine and total hip.
Negatively correlated with BMD, sBAP, sOC, and sNTX may be sensitive and relatively specific markers to evaluate age-related changes of bone turnover.
研究健康男性中骨形成标志物即血清骨碱性磷酸酶（sBAP）和血清骨钙素（sOC）、骨吸收标志物即Ⅰ型胶原交联N端肽（sNTX）以及骨密度（BMD）随年龄的变化情况。
采用酶联免疫吸附法（ELISA）检测389名年龄在20 - 80岁之间、均为汉族的随机选取男性的血清sBAP、sOC和sNTX。使用双能X线骨密度仪测量腰椎、左侧股骨颈、Ward三角区和髋部的骨密度。分析这些标志物与年龄的关系。
（1）sBAP、sOC和sNTX与年龄呈负相关，与其他回归模型相比，三次回归模型能更好地反映骨生化标志物的年龄相关变化（R² = 0.013 - 0.029，P < 0.05）；（2）当所有受试者按10岁分层时，骨生化标志物值在20 - 29岁年龄组最高，sBAP为30.9 U/L ± 12.6 U/L，sOC为12.6 μg/L ± 6.2 μg/L，sNTX为18.2 μmol/L ± 6.6 μmol/L；随后随年龄增长而下降，在50 - 59岁年龄组降至最低点，sBAP为26.9 U/L ± 8.6 U/L，sOC为9.2 μg/L ± 5.3 μg/L，sNTX为15.6 μmol/L ± 6.1 μmol/L。60岁以后，sNTX略有升高至16.0 μmol/L ± 6.1 μmol/L，然而，BAP和sOC保持稳定；（3）在调整年龄、身高、体重、BMI和吸烟因素后，血清BAP与多个骨骼部位的骨密度呈负相关。sOC与除脊柱外侧外的多个骨骼部位的骨密度呈负相关；sNTX与腰椎和全髋部的骨密度呈负相关。
sBAP、sOC和sNTX与骨密度呈负相关，可能是评估骨转换年龄相关变化的敏感且相对特异的标志物。
