Peixinho C, Tavares P, Tomáz M R, Taborda-Barata L, Tomáz C
Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal.
Allergol Immunopathol (Madr). 2006 Sep-Oct;34(5):206-11. doi: 10.1157/13094028.
Differences in latex allergen sensitization profiles have been described between children undergoing repeated surgical interventions and health care workers. The purpose of this study was to determine whether such sensitization profiles are associated with differences in the expression of latex allergen between the internal and external surfaces of surgical gloves.
Extracts were obtained from whole surgical gloves as well as from their external and internal surfaces. The extracts were centrifuged, filtered, concentrated, dialyzed and lyophilized. The protein profile of the extracts was analyzed using hydrophobic interaction chromatography (HIC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immunoblotting was performed using sera from two patients with confirmed latex allergy. Latex recombinant allergen-specific IgE in these two patients was determined using a fluorescence enzyme immunoassay (FEIA) method. Latex allergen quantification was determined on both glove surfaces using an ELISA method.
HIC and SDS-PAGE showed qualitative and quantitative differences in proteins between the internal and external glove surfaces, with the former being much richer in proteins. Immunoblotting of glove extracts using sera from two latex-allergic health workers showed differences between glove surface extracts. ELISA quantification of latex allergens demonstrated that the internal glove surface had high amounts of Hev b 5 and Hev b 6.02 whereas the external surface showed Hev b 1, Hev b 3, and Hev b 6.02.
Our results reveal substantial differences in the composition of latex allergen profiles between the internal and external surfaces of surgical latex gloves, which may suggest a relationship between latex allergen localization and sensitization routes in different risk groups.
已报道接受重复外科手术干预的儿童与医护人员在乳胶过敏原致敏情况方面存在差异。本研究的目的是确定这种致敏情况是否与手术手套内外表面乳胶过敏原表达的差异有关。
从完整的手术手套及其内外表面获取提取物。提取物经离心、过滤、浓缩、透析和冻干处理。使用疏水相互作用色谱法（HIC）和十二烷基硫酸钠聚丙烯酰胺凝胶电泳法（SDS-PAGE）分析提取物的蛋白质谱。使用两名确诊为乳胶过敏患者的血清进行免疫印迹。采用荧光酶免疫测定法（FEIA）测定这两名患者中乳胶重组过敏原特异性IgE。使用酶联免疫吸附测定法（ELISA）对两种手套表面的乳胶过敏原进行定量。
HIC和SDS-PAGE显示手套内外表面蛋白质在定性和定量上存在差异，内表面蛋白质含量更丰富。使用两名乳胶过敏医护人员的血清对手套提取物进行免疫印迹，显示手套表面提取物之间存在差异。ELISA对乳胶过敏原的定量分析表明，手套内表面含有大量的Hev b 5和Hev b 6.02，而外表面含有Hev b 1、Hev b 3和Hev b 6.02。
我们的结果揭示了外科乳胶手套内外表面乳胶过敏原谱组成存在显著差异，这可能表明乳胶过敏原定位与不同风险组致敏途径之间存在关联。
