Hev B 6.02 是职业性接触天然胶乳手套的医护人员致敏的最重要过敏原。

Hev B 6.02 is the most important allergen in health care workers sensitized occupationally by natural rubber latex gloves.

机构信息

Department of Allergy and Immunology, National Research Institute for Child Health & Development, Tokyo, Japan.

出版信息

Allergol Int. 2009 Sep;58(3):347-55. doi: 10.2332/allergolint.08-OA-0046. Epub 2009 May 25.

DOI:10.2332/allergolint.08-OA-0046

PMID:19454841

Abstract

BACKGROUND

Natural rubber latex (NRL) allergy is a common occupational disease in health care workers (HCW). However, few reports have compared the major allergen of HCWs to those in gloves that are routinely used in the hospital. The aim of this study was to evaluate the major NRL allergens in gloves used by HCWs.

METHODS

We studied 20 HCWs who were suspected to have latex allergy (LA). We performed a skin prick test (SPT) using NRL allergens. Serological testing was performed using the ImmunoCAPTM. The total amount of protein and the antigenic protein concentrations extracted from NRL gloves were measured. Four different types of FITkitTM were used to measure the concentrations of Hev b 1, 3, 5, and 6.02 in the gloves.

RESULTS

A SPT using NRL extract identified 14 cases with positive reactions. The sensitivity and specificity of the SPT scores to the NRL glove extract were 100%. The sensitivity of latex specific IgE was 100% but the specificity was 14.2%. The sensitivity and specificity of rHev b 6.02 specific IgE were 100% in the LA group. The total amounts of protein from the medical gloves for surgery and examination were 265 microg/g and 95 microg/g, respectively. The antigenic protein concentrations in the gloves were 24.9 microg/g and 1.0 microg/g, respectively. The total amounts of the specific four allergens in the NRL gloves were 2.18 microg/g and 0.45 microg/g, respectively.

CONCLUSIONS

We concluded that the main allergen of HCWs who have been sensitized occupationally by NRL gloves was Hev b 6.02.

摘要

背景

天然橡胶乳胶（NRL）过敏是医护人员（HCW）中常见的职业病。然而，很少有报道比较 HCW 中主要过敏原与医院常规使用的手套中的主要过敏原。本研究旨在评估 HCW 使用的手套中的主要 NRL 过敏原。

方法

我们研究了 20 名疑似乳胶过敏（LA）的 HCW。我们使用 NRL 过敏原进行皮肤点刺试验（SPT）。使用 ImmunoCAPTM 进行血清学检测。测量从 NRL 手套中提取的总蛋白量和抗原蛋白浓度。使用四种不同类型的 FITkitTM 测量手套中 Hev b 1、3、5 和 6.02 的浓度。

结果

使用 NRL 提取物进行 SPT 鉴定出 14 例阳性反应。SPT 评分对 NRL 手套提取物的敏感性和特异性均为 100%。乳胶特异性 IgE 的敏感性为 100%，但特异性为 14.2%。LA 组 rHev b 6.02 特异性 IgE 的敏感性和特异性均为 100%。手术和检查用医用手套的总蛋白量分别为 265μg/g 和 95μg/g，抗原蛋白浓度分别为 24.9μg/g 和 1.0μg/g。NRL 手套中四种特定过敏原的总量分别为 2.18μg/g 和 0.45μg/g。