Hoess A, Teuscher N, Thormann A, Aurich H, Heilmann A
Fraunhofer Institute for Mechanics of Materials, Heideallee 19, D-06120 Halle, Germany.
Acta Biomater. 2007 Jan;3(1):43-50. doi: 10.1016/j.actbio.2006.07.007. Epub 2006 Oct 24.
Nanoporous aluminum oxide membranes were prepared by anodic oxidation of aluminum for application as novel cell culture substrates. Self-supporting as well as mechanically stabilized nanoporous membranes were produced from aluminum plates and micro-imprinted aluminum foils, respectively. Membranes of two different pore sizes (70 and 260 nm) were selected to investigate cellular interactions with such nanoporous substrates using cells of hepatoma cell line HepG2. The membranes express excellent cell-growth conditions. As shown by scanning electron microscopy investigations, the cells could easily adhere to the membranes and proliferate during a 4 day cell culture period. The cells exhibit normal morphology and were able to penetrate into pores with a diameter of 260 nm by small extensions (filopodia). On mechanically stabilized aluminum oxide membranes it was observed that the cells even adhere to the walls of the small cavities. It was demonstrated experimentally that the nanoporous aluminum oxide membranes are well suited as substrates in cell culture model systems for metabolic, pharmacological/toxicological research, tissue engineering and studies on pathogens as well as bioartificial liver systems.
通过铝的阳极氧化制备了纳米多孔氧化铝膜，用作新型细胞培养底物。分别从铝板和微压印铝箔制备了自支撑和机械稳定的纳米多孔膜。选择两种不同孔径（70和260nm）的膜，使用肝癌细胞系HepG2细胞研究细胞与此类纳米多孔底物的相互作用。这些膜表现出优异的细胞生长条件。扫描电子显微镜研究表明，细胞在4天的细胞培养期内能够轻松粘附在膜上并增殖。细胞呈现正常形态，能够通过小的延伸（丝状伪足）穿透到直径为260nm的孔中。在机械稳定的氧化铝膜上观察到细胞甚至粘附在小腔的壁上。实验证明，纳米多孔氧化铝膜非常适合作为细胞培养模型系统中的底物，用于代谢、药理/毒理学研究、组织工程以及病原体研究和生物人工肝系统。
