Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Korea.
Nanotechnology. 2010 Mar 26;21(12):125104. doi: 10.1088/0957-4484/21/12/125104. Epub 2010 Mar 2.
We systematically analyzed the adhesion and the proliferation of cells on various nanoporous alumina surfaces to understand the effects of nanostructured surfaces on cell behavior. Various nanoporous surfaces were fabricated using the anodizing method and characterized by atomic force microscopy and scanning electron microscopy. The adhesion rate and proliferation rate of cells as functions of pore size and depth were statistically investigated using a colorimetric method. The adhesion rate of cells was not affected by the depth of the nanoporous surface whereas the proliferation of cells dramatically increased when the aspect ratio of the nanopore was near unity. This phenomenon was further verified by comparing the change in roughness of the cytoplasmic layer of cells adhered on a nanoporous surface with that of a bare nanoporous surface. The proliferation of cells was also influenced by the pore size of the nanoporous surface because the nanostructure could control the interaction between extracellular matrix (ECM) molecules and the surface. In conclusion, the nanostructured surfaces affected cell adhesion and proliferation by increasing the surface area to which the cells could adhere, and the interactions between small ECM molecules were influenced by the sufficiently small structures of the nanosurface.
我们系统地分析了细胞在各种纳米多孔氧化铝表面上的黏附和增殖情况，以了解纳米结构化表面对细胞行为的影响。采用阳极氧化法制备了各种纳米多孔表面，并通过原子力显微镜和扫描电子显微镜进行了表征。使用比色法对细胞的黏附率和增殖率作为孔径和深度的函数进行了统计研究。细胞的黏附率不受纳米多孔表面深度的影响，而当纳米孔的纵横比接近 1 时，细胞的增殖率显著增加。通过比较黏附在纳米多孔表面上的细胞质层的粗糙度与裸露纳米多孔表面的粗糙度的变化，进一步验证了这一现象。细胞的增殖也受到纳米多孔表面孔径的影响，因为纳米结构可以控制细胞外基质（ECM）分子与表面之间的相互作用。总之，纳米结构化表面通过增加细胞可以黏附的表面积来影响细胞的黏附和增殖，而小的 ECM 分子之间的相互作用则受到纳米表面的足够小结构的影响。
