通过毛细管电泳-质谱联用技术分析细胞内核苷酸

Analysis of intracellular nucleotides by capillary electrophoresis-mass spectrometry.

作者信息

Friedecký D, Bednár P, Procházka M, Adam T

机构信息

Laboratory for Inherited Metabolic Disorders, Department of Clinical Biochemistry, University Hospital Olomouc, Olomouc, Czech Republic.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2006;25(9-11):1233-6. doi: 10.1080/15257770600894667.

DOI:10.1080/15257770600894667

PMID:17065097

Abstract

A pilot study using capillary electrophoresis with mass spectrometry for the analysis of nucleotides in human erythrocytes is presented. Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside in order to mimic situation in defect of purine metabolism--AICA-ribosiduria. Characteristic AICA-ribotides together with normal nucleotides were separated by capillary electrophoresis in acetate buffer (20 mmol/L, pH 4.4) and identified on line by mass spectrometry.

摘要

本文介绍了一项使用毛细管电泳-质谱联用技术分析人红细胞中核苷酸的初步研究。将红细胞与5-氨基-4-咪唑甲酰胺核苷一起孵育，以模拟嘌呤代谢缺陷——AICA-核糖苷尿症的情况。在醋酸盐缓冲液（20 mmol/L，pH 4.4）中通过毛细管电泳分离特征性AICA-核糖核苷酸和正常核苷酸，并通过质谱进行在线鉴定。