Soga Tomoyoshi
Institute for Advanced Biosciences, Keio University and Human Metabolome Technologies Inc., Yamagata, Japan.
Methods Mol Biol. 2007;358:129-37. doi: 10.1007/978-1-59745-244-1_8.
A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis-mass spectrometry (CE-MS) is described. Metabolites are first separated by CE based on charge and size, and then selectively detected using MS by monitoring ions over a large range of m/z values. This technique enables the simultaneous determination of over 1000 charged species, and it can readily be applied to various types of biological samples originating from bacteria, plants, mammals, body fluids, and others. This chapter highlights detailed practical procedures for using this technology.
本文描述了一种通过毛细管电泳-质谱联用（CE-MS）对带电代谢物进行全面定量分析的新方法。代谢物首先通过CE根据电荷和大小进行分离，然后通过在大范围的m/z值上监测离子，使用MS进行选择性检测。该技术能够同时测定1000多种带电物质，并且可以很容易地应用于源自细菌、植物、哺乳动物、体液等的各种类型的生物样品。本章重点介绍了使用该技术的详细实际操作步骤。
