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Determination of thymidine phosphorylase activity in human blood cells and fibroblasts by a nonradiochemical assay using reversed-phase high-performance liquid chromatography.

作者信息

van Kuilenburg A B P, Zoetekouw L

机构信息

Academic Medical Center, Emma Children's Hospital and Department of Clinical Chemistry, University of Amsterdam, Amsterdam, The Netherlands.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2006;25(9-11):1261-4. doi: 10.1080/15257770600889006.

DOI:10.1080/15257770600889006
PMID:17065103
Abstract

In this study, we demonstrated that the highest activity of thymidine phosphorylase (TP) was found in peripheral blood mononuclear (PBM) cells followed by that of thrombocytes and granulocytes whereas no activity of TP could be detected in erythrocytes. The activity of TP in leukocytes proved to be intermediate compared to the TP activity observed in PBM cells and granulocytes. The activity of TP also was readily detectable in human fibroblasts.

摘要

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