Schaeffer E L, Gattaz W F
Laboratory of Neuroscience (LIM-27), Department and Institute of Psychiatry, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
J Neural Transm (Vienna). 2007 Mar;114(3):379-85. doi: 10.1007/s00702-006-0585-4. Epub 2006 Oct 27.
In rats, the inhibition of phospholipase A(2) (PLA(2)) in hippocampus was reported to impair memory acquisition. In the present study we investigated in rats whether PLA(2) inhibition in hippocampus is also related to impairment of memory retrieval. Rats were bilaterally implanted with cannulae in hippocampal CA1 region. After recovery, animals were submitted to one-trial step-down inhibitory avoidance task and tested for long-term memory (LTM) 24 h later. Before test session, animals received infusions of vehicle or the PLA(2) inhibitor PACOCF(3). Inhibition of PLA(2) activity impaired LTM retrieval. Memory impairment was fully reversed once PLA(2) activity was recovered. Moreover, LTM retrieval per se increased PLA(2) activity. To our knowledge, we demonstrated for the first time that PLA(2) activity is required for memory retrieval. Because reduced PLA(2) activity has been found in Alzheimer's disease brains, the present results may be relevant to clarify at least part of the biology of this disorder.
据报道，在大鼠中，海马体中磷脂酶A2（PLA2）的抑制会损害记忆获取。在本研究中，我们在大鼠中研究了海马体中PLA2的抑制是否也与记忆检索受损有关。大鼠双侧海马CA1区植入套管。恢复后，动物接受单次递减抑制性回避任务，并在24小时后测试长期记忆（LTM）。在测试前，动物接受载体或PLA2抑制剂PACOCF3的输注。PLA2活性的抑制损害了LTM检索。一旦PLA2活性恢复，记忆障碍就完全逆转。此外，LTM检索本身会增加PLA2活性。据我们所知，我们首次证明了PLA2活性是记忆检索所必需的。由于在阿尔茨海默病大脑中发现PLA2活性降低，目前的结果可能有助于阐明这种疾病生物学的至少一部分。
