Houserová Pavlína, Kubán Petr, Kubán Vlastimil
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic.
Electrophoresis. 2006 Nov;27(22):4508-15. doi: 10.1002/elps.200600274.
Capillary electrophoretic separation of four mercury species (inorganic Hg(2+), methyl-, ethyl-, and phenylmercury) was achieved in less than 8 min with an electrolyte consisting of 150 mM 2-amino-2-methyl-propanol (AMP) at pH 11.6. The analytes were complexed with 0.1% mercaptopropionic acid (MPA), separated in counter-EOF as anionic complexes, and detected by a contactless conductometric detector. Ion exchange preconcentration with on-column formation of MPA-mercury complexes was developed. Preconcentration factors of 25-150 were achieved and LODs in the range of 2.9-6.9 ng/mL were feasible. This method may prove to be applicable as a rapid screening method for mercury speciation in environmental samples.
采用由150 mM 2-氨基-2-甲基-1-丙醇（AMP）组成、pH值为11.6的电解质，在不到8分钟的时间内实现了四种汞形态（无机汞（Hg(2+)）、甲基汞、乙基汞和苯基汞）的毛细管电泳分离。分析物与0.1%的巯基丙酸（MPA）络合，作为阴离子络合物在反向电渗流中分离，并通过非接触式电导检测器进行检测。开发了柱上形成MPA-汞络合物的离子交换预富集方法。实现了25至150的预富集因子，检测限在2.9至6.9 ng/mL范围内是可行的。该方法可能被证明适用于环境样品中汞形态的快速筛选方法。
