Kubán Petr, Houserová Pavlína, Kubán Pavel, Hauser Peter C, Kubán Vlastimil
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic.
J Sep Sci. 2007 May;30(7):1070-6. doi: 10.1002/jssc.200600476.
A capillary electrophoretic method for the separation of four mercury species with amperometric detection was developed. Inorganic Hg2+, methyl-, ethyl-, and phenyl-mercury were complexed with L-cysteine and separated in a counterelectroosmotic mode in an electrolyte solution comprised of 20 mM sodium tetraborate at pH 9.5. Amperometric detection of separated species was achieved at passivated copper electrode under electrocatalytic oxidation conditions. The four mercury species were separated in less than 8 min with LODs ranging from 170 to 450 microg/L. Cation exchange preconcentration was used to decrease the LODs down to 1.7 microg/L.
开发了一种采用安培检测法分离四种汞形态的毛细管电泳方法。无机汞离子（Hg2+）、甲基汞、乙基汞和苯基汞与L-半胱氨酸络合，并在pH 9.5的20 mM四硼酸钠组成的电解质溶液中以反向电渗流模式进行分离。在电催化氧化条件下，在钝化铜电极上实现了对分离后汞形态的安培检测。四种汞形态在不到8分钟内分离，检测限范围为170至450 μg/L。采用阳离子交换预富集将检测限降低至1.7 μg/L。
