Kilgour R D, Gardiner K, Gardiner P F
Department of Exercise Science, Concordia University, Montréal, Qué., Canada.
Can J Physiol Pharmacol. 1990 Oct;68(10):1317-21. doi: 10.1139/y90-198.
Phosphofructokinase activity was measured in the sciatic nerve of streptozotocin-induced diabetic and nondiabetic rats. Average steady-state phosphofructokinase activity was obtained from three consecutive segments of the mid-femoral region in the left sciatic nerve in both diabetic (4 and 24 weeks) and nondiabetic, age-matched animals. Over time, phosphofructokinase activity significantly decreased (p less than 0.05) with diabetes, with no effect demonstrated within similar age-groups. The accumulation of phosphofructokinase activity was accomplished by ligating the mid-femoral region of the right sciatic nerve for 24 h. Anterograde and retrograde axonal transport of phosphofructokinase was measured in the 3-mm segment proximal and distal to the ligature, respectively. There was a trend (p = 0.0627) towards a decline in net proximal accumulation (mean proximal minus mean background) with age. Net distal (mean distal minus mean background) activity declined by 80% (p less than 0.05) in the control group between 4 and 24 weeks of the diabetic state. However, diabetic animals did not experience the same age-related decline in retrograde transport. The findings suggest that diabetes affects the age-associated evolution of retrograde transport, presumably a reflection of the neuropathy occurring in the distal axon branches, without altering anterograde transport to any appreciable extent.
在链脲佐菌素诱导的糖尿病大鼠和非糖尿病大鼠的坐骨神经中测量了磷酸果糖激酶活性。从糖尿病（4周和24周）和年龄匹配的非糖尿病动物的左坐骨神经中股中部区域的三个连续节段获得平均稳态磷酸果糖激酶活性。随着时间的推移，糖尿病状态下磷酸果糖激酶活性显著降低（p<0.05），在相似年龄组内未显示出影响。通过结扎右坐骨神经的中股区域24小时来实现磷酸果糖激酶活性的积累。分别在结扎处近端和远端的3毫米节段中测量磷酸果糖激酶的顺行和逆行轴突运输。随着年龄增长，近端净积累（平均近端减去平均背景）有下降趋势（p = 0.0627）。在糖尿病状态的4周和24周之间，对照组的远端净活性（平均远端减去平均背景）下降了80%（p<0.05）。然而，糖尿病动物在逆行运输中没有经历相同的与年龄相关的下降。这些发现表明，糖尿病影响逆行运输与年龄相关的演变，这可能反映了远端轴突分支中发生的神经病变，而不会在任何明显程度上改变顺行运输。
