Calcutt N A, Willars G B, Tomlinson D R
Department of Physiology and Pharmacology, Medical School, University of Nottingham, UK.
Muscle Nerve. 1988 Dec;11(12):1206-10. doi: 10.1002/mus.880111204.
This study examined the anterograde axonal transport of activities of the cytoplasmic enzymes choline acetyltransferase and 6-phosphofructokinase in genetically diabetic C57BL/Ks (db/db) mice and their nondiabetic (+/?) littermates. Diabetic mice exhibited marked reductions in the accumulation of both choline acetyltransferase and 6-phosphofructokinase activity against a constriction of the left sciatic nerve (38% and 51% of nondiabetic values, respectively). Enzyme activities per unit length of unconstricted nerve were not different from those of nondiabetic mice. The nerves of diabetic mice did not accumulate measurable amounts of sorbitol or fructose and showed no myo-inositol depletion. Thus this study concludes that, in diabetic mice, the deficits in anterograde axonal transport of these two enzymes do not arise from the accumulation of sorbitol and fructose nor from depletion of nerve free myo-inositol.
本研究检测了遗传性糖尿病C57BL/Ks（db/db）小鼠及其非糖尿病（+/？）同窝仔鼠中细胞质酶胆碱乙酰转移酶和6-磷酸果糖激酶活性的顺行轴突运输情况。糖尿病小鼠在左侧坐骨神经受压后，胆碱乙酰转移酶和6-磷酸果糖激酶活性的积累均显著降低（分别为非糖尿病小鼠值的38%和51%）。未受压神经单位长度的酶活性与非糖尿病小鼠无异。糖尿病小鼠的神经未积累可测量量的山梨醇或果糖，且未出现肌醇耗竭。因此，本研究得出结论，在糖尿病小鼠中，这两种酶顺行轴突运输的缺陷并非由山梨醇和果糖的积累或神经游离肌醇的耗竭引起。
