Marinucci Lorella, Balloni Stefania, Becchetti Ennio, Belcastro Salvatore, Guerra Mario, Calvitti Mario, Lilli Cinzia, Calvi Edoardo Maria, Locci Paola
Department of Experimental Medicine and Biochemical Science, University of Perugia, Italy.
Int J Oral Maxillofac Implants. 2006 Sep-Oct;21(5):719-25.
Cell proliferation and extracellular matrix formation are primary events in bone formation. At the dental implant-tissue interface, implant surface roughness modulates osteoblast functions. The aim of the present in vitro study was to investigate the effect of varying surface roughness of titanium implant material on cell proliferation and mRNA expression of specific markers of osteoblast phenotype.
Primary cultures of osteoblasts derived from human mandibular bone were cultured on titanium surfaces. Three titanium surfaces were studied: machined titanium, microsandblasted titanium, and macro-sandblasted titanium (average surface roughnesses of 0.5 and 3 microm, respectively). Cell morphology was estimated by scanning electron microscope analysis and cell proliferation by measuring the amount of 3H-thymidine incorporation into DNA. mRNA expression of osteonectin, osteopontin, bone sialoprotein (BSP), and Runx2, which are markers of osteoblastic phenotype, were determined by reverse transcriptase polymerase chain reaction (RT-PCR) analysis.
Human osteoblasts cultured on machined titanium spread more and were flatter than cells cultured on rough titanium. All blasted surfaces showed significantly higher DNA synthesis than the machined surfaces. Osteonectin mRNA expression was similar on all surfaces. Other mRNA transcripts were increased in osteoblasts cultured on rough titanium surfaces, particularly the macrosandblasted surface.
An average surface roughness of 3 microm (macro-sandblasted titanium) is more suitable than an average surface roughness of 0.5 microm (micro-sandblasted titanium) in favoring osteoblast differentiation in vitro.
细胞增殖和细胞外基质形成是骨形成的主要过程。在牙种植体与组织的界面处，种植体表面粗糙度会调节成骨细胞的功能。本体外研究的目的是调查钛种植体材料不同的表面粗糙度对细胞增殖和成骨细胞表型特异性标志物的mRNA表达的影响。
将源自人下颌骨的成骨细胞原代培养物培养在钛表面上。研究了三种钛表面：机械加工钛、微喷砂钛和宏喷砂钛（平均表面粗糙度分别为0.5微米和3微米）。通过扫描电子显微镜分析评估细胞形态，并通过测量掺入DNA中的3H-胸腺嘧啶核苷的量来评估细胞增殖。通过逆转录聚合酶链反应（RT-PCR）分析确定骨粘连蛋白、骨桥蛋白、骨唾液蛋白（BSP）和Runx2的mRNA表达，这些是成骨细胞表型的标志物。
在机械加工钛上培养的人成骨细胞比在粗糙钛上培养的细胞铺展得更多且更扁平。所有喷砂表面的DNA合成均显著高于机械加工表面。所有表面上骨粘连蛋白mRNA表达相似。在粗糙钛表面（特别是宏喷砂表面）上培养的成骨细胞中，其他mRNA转录物增加。
平均表面粗糙度为3微米（宏喷砂钛）比平均表面粗糙度为0.5微米（微喷砂钛）更有利于体外成骨细胞分化。
