Isa Zakiah M, Schneider Galen B, Zaharias Rebecca, Seabold Denise, Stanford Clark M
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Int J Oral Maxillofac Implants. 2006 Mar-Apr;21(2):203-11.
The objective of this study was to test the hypothesis that fluoride-modified titanium surfaces would enhance osteoblast differentiation. Osteoblast growth on a moderately rough etched fluoride-modified titanium surface (alteration in cellular differentiation) was compared to osteoblast growth on the same surface grit-blasted with titanium dioxide. The potential role of nanometer-level alterations on cell shape and subsequent differentiation was then compared.
Human embryonic palatal mesenchymal (HEPM) cultures were incubated on the respective surfaces for 1, 3, and 7 days, followed by analysis for cell proliferation, alkaline phosphatase (ALP) -specific activity, and mRNA steady-state expression for bone-related genes (ALP, type I collagen, osteocalcin, bone sialoprotein [BSP] II, Cbfa1, and osterix) by real-time polymerase chain reaction (PCR).
The different surfaces did not alter the mRNA expression for ALP, type I collagen, osterix, osteocalcin, or BSP II. However, Cbfa1 expression on the fluoride-modified titanium surface was significantly higher (P < .001) at 1 week. The number of cells on this surface was 20% lower than the number of cells on the surface TiO2-blasted with 25-microm particles but not significantly different from the number of cells on the surface TiO2-blasted with 125-microm particles. Cells grown on all the titanium surfaces expressed similar levels of ALP activity.
The results indicated that a fluoride-modified surface topography, in synergy with surface roughness, may have a greater influence on the level of expression of Cbfa1 (a key regulator for osteogenesis) than the unmodified titanium surfaces studied.
本研究的目的是验证氟改性钛表面会增强成骨细胞分化这一假设。将成骨细胞在适度粗糙的蚀刻氟改性钛表面（细胞分化改变）上的生长情况与在经二氧化钛喷砂处理的相同表面粗糙度的钛表面上的成骨细胞生长情况进行比较。然后比较纳米级改变对细胞形状及后续分化的潜在作用。
将人胚胎腭间充质（HEPM）培养物在各自表面上培养1、3和7天，随后通过实时聚合酶链反应（PCR）分析细胞增殖、碱性磷酸酶（ALP）特异性活性以及与骨相关基因（ALP、I型胶原、骨钙素、骨唾液蛋白[BSP]II、Cbfa1和osterix）的mRNA稳态表达。
不同表面并未改变ALP、I型胶原、osterix、骨钙素或BSP II的mRNA表达。然而，在1周时，氟改性钛表面上的Cbfa1表达显著更高（P <.001）。该表面上的细胞数量比用25微米颗粒喷砂处理的TiO₂表面上的细胞数量低20%，但与用125微米颗粒喷砂处理的TiO₂表面上的细胞数量无显著差异。在所有钛表面上生长的细胞表达的ALP活性水平相似。
结果表明，与未改性的钛表面相比，氟改性的表面形貌与表面粗糙度协同作用，可能对Cbfa1（骨生成的关键调节因子）的表达水平有更大影响。
