Il'ina A V, Mestechkina N M, Shcherbukhin V D, Varlamov V P
Prikl Biokhim Mikrobiol. 2006 Sep-Oct;42(5):580-6.
The depolymerisation of legume seed Galactomannans by commercial preparation "Celloviridin G20x" to produce fragments of macromolecules with unchanged monomer ratio was studied. The hydrolysis of four galactomannans in whole range of monomer ratio for this phytopolysaccharide group 1.1-5.1 was performed by enzyme preparation, which is a sum of carbohydrases (producent--Trichoderma reesei). Degree of substitution 1,4-beta-D-mannopyranose chain on C-6 position by alpha-galactose residues had influence on values of molecular mass of final reaction products. The fragments with high yelds and monomer ratio nearly of that initial polysaccharides were obtained.
研究了用商业制剂“Celloviridin G20x”使豆科植物种子半乳甘露聚糖解聚，以产生单体比例不变的大分子片段。通过酶制剂对该植物多糖组单体比例范围为1.1 - 5.1的四种半乳甘露聚糖进行水解，该酶制剂是多种碳水化合物酶的总和（生产商——里氏木霉）。C - 6位上1,4 - β - D - 甘露吡喃糖链被α - 半乳糖残基取代的程度对最终反应产物的分子量值有影响。获得了高产量且单体比例与初始多糖相近的片段。
