Pawar Harshal A, Lalitha K G
Research Scholar, Ultra College of Pharmacy, 4/235, College Road, Thasildar Nagar, Madurai 625020, Tamil Nadu, India.
Professor and Head, Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, 4/235, College Road, Thasildar Nagar, Madurai 625020, Tamil Nadu, India.
Int J Biol Macromol. 2014 Apr;65:167-75. doi: 10.1016/j.ijbiomac.2014.01.026. Epub 2014 Jan 20.
Seed galactomannans are neutral, heterogeneous polysaccharides widely distributed in nature. The Mannose/Galactose ratios differ from gum to gum, resulting in a change in structure, which in turn, determines the various industrial applications of seed galactomannans. Senna tora (Family: Fabaceae) is a fast growing and spreading under shrub of which seeds, pods and leaves are extensively used for medicinal applications. The seeds have been found to be an alternative source of commercial gums. The present investigation deals with isolation, purification and characterization of galactomannans from the seeds of Senna tora (S. tora). The galactomannan extraction was based on mechanical separation of the endosperm, water dissolution, centrifugation and precipitation with acetone. The polysaccharide obtained from S. tora seeds was characterized by using physicochemical and chromatographic procedures, as well as FTIR, Mass, (13)C NMR and (1)H NMR spectroscopy. The results indicated that the gum has the basic structure of galactomannans with a main chain of (1→4)-linked β-D-mannopyranosyl units to which single α-(1→6)-D-linked galactopyranosyl units are attached through block pattern. The rheological studies indicated that the S. tora gum (1%, w/w) solution possesses pseudoplastic flow. The viscosity and other rheological properties confirmed its suitability as an excipient in the development of sustained release delivery systems.
种子半乳甘露聚糖是一类中性、异质的多糖，广泛分布于自然界。不同种子胶的甘露糖/半乳糖比例各异，导致结构有所变化，进而决定了种子半乳甘露聚糖的各种工业应用。望江南（豆科）是一种生长迅速且蔓延的低矮灌木，其种子、豆荚和叶子被广泛用于医药用途。已发现种子是商业胶的替代来源。本研究涉及从望江南种子中分离、纯化和表征半乳甘露聚糖。半乳甘露聚糖的提取基于胚乳的机械分离、水溶解、离心以及用丙酮沉淀。通过物理化学和色谱方法以及傅里叶变换红外光谱（FTIR）、质谱、碳 - 13核磁共振（¹³C NMR）和氢 - 1核磁共振（¹H NMR）光谱对望江南种子获得的多糖进行了表征。结果表明，该胶具有半乳甘露聚糖的基本结构，其主链由（1→4）连接的β - D - 甘露吡喃糖基单元组成，单个α - （1→6） - D - 连接的半乳吡喃糖基单元以嵌段模式连接在主链上。流变学研究表明，望江南胶（1%，w/w）溶液具有假塑性流动。其粘度和其他流变学性质证实了它作为缓释给药系统开发中辅料的适用性。
