Rodrigues Sandra C G, Maruniak James E
Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611-0620, USA.
J Am Mosq Control Assoc. 2006 Sep;22(3):557-60. doi: 10.2987/8756-971X(2006)22[557:BMIFMC]2.0.CO;2.
Outbreaks of West Nile virus on a Florida alligator farm prompted an investigation of which species of mosquitoes were feeding on the animals at the farm. Mosquitoes were collected on 4 separate overnight trips in September and October 2003 by using CO2-baited Centers for Disease Control light traps and wooden resting boxes that were placed inside or near the alligator housing pens. Mosquitoes were identified to species, bloodfed individuals were separated, and their abdomens were removed for DNA extraction. The DNA was tested to determine the vertebrate origin of the blood meal by polymerase chain reaction (PCR) amplification by using 4 primer sets specific to crocodilians, alligators, mammals, and birds. PCR products were sequenced to identify hosts. Of the 37 mosquito blood meals tested, 13 blood meals were positively identified to species, and 7 blood meals of those 13 were from Alligator mississippiensis, the American alligator. Alligator blood was found in Culex erraticus, Mansonia dyari, and Ma. titillans, and to our knowledge, this represents the first report of these mosquito species feeding on American alligators.
佛罗里达州一个短吻鳄养殖场爆发西尼罗河病毒，促使人们对该养殖场中叮咬这些动物的蚊子种类展开调查。2003年9月和10月期间，在4次不同的夜间行动中，使用美国疾病控制中心的二氧化碳诱捕灯诱捕器和放置在短吻鳄饲养围栏内或附近的木制栖息箱收集蚊子。蚊子被鉴定到种类，吸食了血液的个体被分离出来，其腹部被移除用于DNA提取。通过使用针对鳄目动物、短吻鳄、哺乳动物和鸟类的4组引物，通过聚合酶链反应（PCR）扩增对DNA进行检测，以确定血餐的脊椎动物来源。对PCR产物进行测序以识别宿主。在检测的37份蚊子血餐中，有13份血餐被明确鉴定到种类，其中这13份中的7份血餐来自密西西比鳄，即美国短吻鳄。在不规律库蚊、迪亚里曼蚊和蒂氏曼蚊中发现了短吻鳄的血液，据我们所知，这是这些蚊子种类吸食美国短吻鳄血液的首次报告。
