Cox L R, Motz J, Troll W, Garte S J
Department of Environmental Medicine, New York University Medical Center, New York 10016.
J Cancer Res Clin Oncol. 1991;117(2):102-8. doi: 10.1007/BF01613132.
Exposure of NIH3T3 cells to retinoic acid resulted in a dose-dependent modulation of transformed focus formation after transfection with an activated H-ras oncogene. Inhibition induced by 10 microM retinoic acid was maximal at 21.4% of control values. Maximal inhibition of transformation was found after exposure to 10 microM retinoic acid between days 0 and 3 of the transfection period. This concentration was also inhibitory for colony formation upon transfection of the non-transforming gene aph, suggesting that retinoic acid acts primarily on the process of transfection to inhibit focus or colony formation. Exposure to retinoic acid during the late period of the transfection protocol (days 14-20) resulted in alterations in focus morphology. A transformed cell line containing H-ras underwent reversion of the transformed phenotype after 4 weeks of treatment with retinoic acid, as determined by alterations in cell morphology and anchorage-independent growth. Phenotypic reversion was not associated with changes in the expression of the exogenous H-ras or endogenous c-myc or c-fos oncogenes.
将NIH3T3细胞暴露于视黄酸中，在用活化的H-ras癌基因转染后，会导致转化灶形成的剂量依赖性调节。10微摩尔视黄酸诱导的抑制作用在对照值的21.4%时达到最大。在转染期的第0天至第3天之间暴露于10微摩尔视黄酸后，发现对转化的最大抑制作用。该浓度对非转化基因aph转染后的集落形成也有抑制作用，这表明视黄酸主要作用于转染过程以抑制灶或集落形成。在转染方案的后期（第14 - 20天）暴露于视黄酸会导致灶形态的改变。通过细胞形态和不依赖贴壁生长的改变确定，含有H-ras的转化细胞系在用视黄酸处理4周后发生了转化表型的逆转。表型逆转与外源性H-ras或内源性c-myc或c-fos癌基因的表达变化无关。
