Yagmurdur H, Ayyildiz A, Karaguzel E, Ogus E, Surer H, Caydere M, Nuhoglu B, Germiyanoglu C
Clinics of Anesthesiology and Reanimation, The Ministry of Health Ankara Research and Training Hospital, Ankara, Turkey.
Acta Anaesthesiol Scand. 2006 Nov;50(10):1238-43. doi: 10.1111/j.1399-6576.2006.01145.x.
Testicular torsion is a urological emergency that requires immediate surgical intervention to prevent testicular damage. The aim of the study was to investigate the preventive effects of thiopental and propofol as anesthetics on testicular ischemia-reperfusion injury.
Forty male Wistar Albino rats were randomly assigned to four groups of 10 rats each. During 5 h, anesthesia was induced and maintained with thiopental in groups 1 and 2 and with propofol in groups 3 and 4. Groups 2 and 4 received left testicular ischemia (torsion) during 1 h and reperfusion (detorsion) during 4 h. Groups 1 and 3 (control groups) had no testicular torsion and detorsion. At the end of 5 h, animals were killed and both ipsilateral and contralateral testes were removed for histopathologic examination and measurement of tissue MDA (malondialdehyde) and NO (nitric oxide) levels.
In the contralateral testes of all the groups, MDA and NO measurements were not different from ipsilateral testes of the control groups. Between the groups 1 and 3, there were no differences in MDA and NO levels. Although torsion/detorsion of testes in group 4 caused significantly increased levels of tissue MDA and NO values compared with group 3, ischemia-reperfusion in group 2 caused a further increase in these levels compared with group 4. The ipsilateral testes in the control groups did not show any morphological changes. Testicular torsion/detorsion in rats with thiopental anesthesia (group 2) caused significantly greater histopathologic injury levels than rats with propofol anesthesia (group 4).
Our results suggest that propofol as an anesthetic agent may prevent testicular damage by scavenging reactive oxygen and nitrogen species and inhibiting lipid peroxidation in an animal model of testicular torsion and detorsion.
睾丸扭转是一种泌尿外科急症，需要立即进行手术干预以防止睾丸损伤。本研究的目的是探讨硫喷妥钠和丙泊酚作为麻醉剂对睾丸缺血再灌注损伤的预防作用。
40只雄性Wistar白化大鼠随机分为4组，每组10只。在5小时内，第1组和第2组用硫喷妥钠诱导并维持麻醉，第3组和第4组用丙泊酚诱导并维持麻醉。第2组和第4组在1小时内进行左侧睾丸缺血（扭转），在4小时内进行再灌注（去扭转）。第1组和第3组（对照组）未进行睾丸扭转和去扭转。5小时结束时，处死动物，取出同侧和对侧睾丸进行组织病理学检查，并测量组织丙二醛（MDA）和一氧化氮（NO）水平。
所有组对侧睾丸的MDA和NO测量值与对照组同侧睾丸无差异。第1组和第3组之间，MDA和NO水平无差异。虽然第4组睾丸扭转/去扭转导致组织MDA水平和NO值显著高于第3组，但第2组的缺血再灌注导致这些水平比第4组进一步升高。对照组同侧睾丸未显示任何形态学变化。硫喷妥钠麻醉的大鼠（第2组）睾丸扭转/去扭转引起的组织病理学损伤水平明显高于丙泊酚麻醉的大鼠（第4组）。
我们的结果表明，在睾丸扭转和去扭转动物模型中，丙泊酚作为麻醉剂可能通过清除活性氧和氮物种以及抑制脂质过氧化来预防睾丸损伤。
