Department of Urology, Ankara Training and Research Hospital, Ankara, Turkey.
Deparment of Pathology Ankara Training Research Hospital, Ankara, Turkey.
Int Braz J Urol. 2018 May-Jun;44(3):617-622. doi: 10.1590/S1677-5538.IBJU.2017.0600.
To investigate the effect of papaverine and alprostadil on testicular torsion-detorsion injury in rats.
A total of 40 male Wistar-Albino rats were used in this study. Four hours of right testicular torsion was applied to each group, excluding sham operated group. The torsion-detorsion (T/D), T/D + papaverine and T/D + alprostadil groups received saline, papaverine and alprostadil at the same time as surgical detorsion, respectively. At 14 days after the surgical detorsion, ischaemic changes and the degree of damage were evaluated with Cosentino scoring and the Johnson tubular biopsy score (JTBS).
JTBS was determined as 8.8±2.7 in the Sham group, 5.08±1.9 in the T/ D+papaverine group, 5.29±2.3 in the T/D +alprostadil group and 2.86±1.9 in the TD group. The JTBS was determined to be statistically significantly high in both the T/D + papaverine group and the T/D + alprostadil group compared to the T/D group (p=0.01, p=0.009). In the T/D + papaverine group, 3 (43 %) testes were classified as Cosentino 2, 3 (43%) as Cosentino 3 and 1 (14 %) as Cosentino 4. In the T/D +alprostadil group, 5 (50 %) testes were classified as Cosentino 2, 3 (30 %) as Cosentino 3 and 2 (20%) as Cosentino 4.
The present study indicated that spermatic cord administration of alprostadil and papaverine showed a protective effect against ischemia/reperfusion injury after right-side testes torsion and histological changes were decreased after testicular ischemia reperfusion injury.
探讨罂粟碱和前列地尔对大鼠睾丸扭转复位损伤的影响。
本研究共使用 40 只雄性 Wistar-Albino 大鼠。每组均进行 4 小时右侧睾丸扭转，不包括假手术组。扭转复位（T/D）、T/D+罂粟碱和 T/D+前列地尔组在手术复位时分别给予生理盐水、罂粟碱和前列地尔。在手术复位后 14 天，用 Cosentino 评分和 Johnson 管状活检评分（JTBS）评估缺血性变化和损伤程度。
Sham 组 JTBS 为 8.8±2.7，T/D+papaverine 组为 5.08±1.9，T/D+alprostadil 组为 5.29±2.3，T/D 组为 2.86±1.9。T/D+papaverine 组和 T/D+alprostadil 组的 JTBS 均明显高于 T/D 组（p=0.01，p=0.009）。在 T/D+papaverine 组中，3 个（43%）睾丸被归类为 Cosentino 2，3 个（43%）为 Cosentino 3，1 个（14%）为 Cosentino 4。在 T/D+alprostadil 组中，5 个（50%）睾丸被归类为 Cosentino 2，3 个（30%）为 Cosentino 3，2 个（20%）为 Cosentino 4。
本研究表明，精索内给予前列地尔和罂粟碱对右侧睾丸扭转后缺血/再灌注损伤具有保护作用，睾丸缺血再灌注损伤后组织学变化减少。
