[一项关于耐药性对人胰腺癌细胞系放射抗性影响的初步研究]

[A pilot study on the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines].

作者信息

Chen Ge, Zhao Yu-pei, Jiang Hua, Guo Jun-chao, Zhang Li-yang, Niu Bei-zhan, Wu Wei-wei

机构信息

Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China.

出版信息

Zhonghua Wai Ke Za Zhi. 2006 Jul 1;44(13):921-3.

PMID:17067488

Abstract

OBJECTIVE

To investigate the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines.

METHODS

Three drug resistant pancreatic cancer cell sublines induced by fluorouracil (5-FU), adriamycin (ADM) and gemcitabine respectively, SW1990/FU, SW1990/ADM and SW1990/Gz, were tested for the cell cycle and radio-sensitivity with flow cytometry and clonogenic assay.

RESULTS

Compared with SW1990, the cell cycle assay indicated higher G(0)/G(1) period percentage in SW1990/FU and SW1990/Gz, but the G(2)/M period percentage decreased; SW1990/FU had the same while SW1990/Gz had lower S period percentage. SW1990/ADM almost had a similar cell cycle with SW1990. Clonogenic assay showed both SW1990/FU and SW1990/Gz had greater survival fraction (SF(2)) than SW1990, but SW1990/ADM had seemingly similar SF(2) as SW1990.

CONCLUSION

Drug resistant pancreatic cancer cell lines have reduced G(2)/M period percentage and increased radioresistance.

摘要

目的

研究耐药性对人胰腺癌细胞系放射抗性的影响。

方法

分别用氟尿嘧啶（5-FU）、阿霉素（ADM）和吉西他滨诱导出三种耐药胰腺癌细胞亚系，即SW1990/FU、SW1990/ADM和SW1990/Gz，采用流式细胞术和克隆形成试验检测其细胞周期和放射敏感性。

结果

与SW1990相比，细胞周期分析显示SW1990/FU和SW1990/Gz的G(0)/G(1)期百分比更高，但G(2)/M期百分比降低；SW1990/FU的S期百分比相同，而SW1990/Gz的S期百分比更低。SW1990/ADM的细胞周期与SW1990几乎相似。克隆形成试验表明，SW1990/FU和SW1990/Gz的存活分数（SF(2)）均高于SW1990，但SW1990/ADM的SF(2)似乎与SW1990相似。