[GCS基因在人胰腺癌细胞SW1990及其耐药亚系中的表达及意义]

[Expression and significance of GCS gene in human pancreatic cancer cell SW1990 and its drug-resistant sublines].

作者信息

Bu Guang-bo, Zhao Yu-pei, Chen Ge, Zhang Tai-ping, Li Li-jun, Wu Yuan-de

机构信息

Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China.

出版信息

Zhonghua Wai Ke Za Zhi. 2006 Oct 1;44(19):1342-4.

PMID:17217822

Abstract

OBJECTIVE

To study the expression and significance of GCS gene in human pancreatic cancer cell line SW1990 and its drug-resistant sublines.

METHODS

SW1990 and its drug-resistant sublines, SW1990/FU, SW1990/ADM and SW1990/GEM were cultured in vitro. CCK-8 (Cell Counting kit-8) was used to detect the drug resistance of the sublines. Relative quantitation of GCS mRNA expression was evaluated by real-time PCR and Western blot was adopted to evaluate the expression of GCS protein.

RESULTS

The drug resistance indexes of SW1990/FU, SW1990/ADM and SW1990/GEM were 339.7, 11.9 and 56.6, respectively. The GCS mRNA and protein were expressed in all SW1990 and its drug-resistant sublines. There was a higher expression of GCS mRNA in all the sublines and a significant difference of GCS protein expression was detected in SW1990/ADM and SW1990/GEM compared with SW1990.

CONCLUSIONS

GCS gene is expressed in SW1990 and its drug-resistance sublines. The high expression of GCS protein in SW1990/ADM and SW1990/GEM might be one reason of resistance to ADM and GEM in the sublines.

摘要

目的

研究GCS基因在人胰腺癌细胞系SW1990及其耐药亚系中的表达及意义。

方法

体外培养SW1990及其耐药亚系SW1990/FU、SW1990/ADM和SW1990/GEM。采用CCK-8（细胞计数试剂盒-8）检测亚系的耐药性。通过实时PCR评估GCS mRNA表达的相对定量，并采用蛋白质免疫印迹法评估GCS蛋白的表达。

结果

SW1990/FU、SW1990/ADM和SW1990/GEM的耐药指数分别为339.7、11.9和56.6。GCS mRNA和蛋白在所有SW1990及其耐药亚系中均有表达。所有亚系中GCS mRNA表达较高，与SW1990相比，SW1990/ADM和SW1990/GEM中GCS蛋白表达有显著差异。