Denda Mitsuhiro, Sokabe Takaaki, Fukumi-Tominaga Tomoko, Tominaga Makoto
Shiseido Research Center, Kanazawa-ku, Yokohama, Japan.
J Invest Dermatol. 2007 Mar;127(3):654-9. doi: 10.1038/sj.jid.5700590. Epub 2006 Oct 19.
Members of the transient receptor potential (TRP) family are temperature sensors, and TRPV1, V3, and V4 are expressed in epidermal keratinocytes. To evaluate the influence of these receptors on epidermal permeability barrier homeostasis, we kept both hairless mouse skin and human skin at various temperatures immediately after tape stripping. At temperatures from 36 to 40 degrees C, barrier recovery was accelerated in both cases compared with the area at 34 degrees C. At 34 or 42 degrees C, barrier recovery was delayed compared with the un-occluded area. 4Alpha-phorbol 12,13-didecanone, an activator of TRPV4, accelerated barrier recovery, whereas ruthenium red, a blocker of TRPV4, delayed barrier recovery. Capsaicin, an activator of TRPV1, delayed barrier recovery, whereas capsazepin, an antagonist of TRPV1, blocked this delay. 2-Aminoethoxydiphenyl borate and camphor, TRPV3 activators, did not affect the barrier recovery rate. As TRPV4 is activated at about 35 degrees C and above, whereas TRPV1 is activated at about 42 degrees C and above, these results suggest that both TRPV1 and TRPV4 play important roles in skin permeability barrier homeostasis. Previous reports suggest the existence of a water flux sensor in the epidermis, and as TRPV4 is known to be activated by osmotic pressure, our results indicate that it might be this sensor.
瞬时受体电位（TRP）家族成员是温度传感器，TRPV1、V3和V4在表皮角质形成细胞中表达。为了评估这些受体对表皮渗透屏障稳态的影响，我们在胶带剥离后立即将无毛小鼠皮肤和人类皮肤置于不同温度下。在36至40摄氏度的温度下，与34摄氏度的区域相比，两种情况下的屏障恢复均加速。在34或42摄氏度时，与未封闭区域相比，屏障恢复延迟。TRPV4激活剂4α-佛波醇12,13-十二烷酮加速了屏障恢复，而TRPV4阻滞剂钌红则延迟了屏障恢复。TRPV1激活剂辣椒素延迟了屏障恢复，而TRPV1拮抗剂辣椒平则阻止了这种延迟。TRPV3激活剂2-氨基乙氧基二苯硼酸和樟脑对屏障恢复率没有影响。由于TRPV4在约35摄氏度及以上被激活，而TRPV1在约42摄氏度及以上被激活，这些结果表明TRPV1和TRPV4在皮肤渗透屏障稳态中均起重要作用。先前的报道表明表皮中存在水通量传感器，并且由于已知TRPV4可被渗透压激活，我们的结果表明它可能就是这种传感器。
