Wang Zhi-qi, Wang Jian-liu, Wei Li-hui, Zhao Dan, Gao Min, Guo Rui-xia
Department of Gynecology, Peking University People's Hospital, Beijing 100044, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Oct 18;38(5):466-9.
To investigate the regulation of 17beta-estradiol on the membrane estrogen receptor alpha.
The Ishikawa cells were treated with 10(-6) mol/L 17beta-estradiol for 0, 3, 10, 30 minutes and 2, 12, 48 hours, respectively. Another group of Ishikawa cells was treated with 10(-12) mol/L, 10(-10) mol/L, 10(-8) mol/L and 10(-6) mol/L 17beta-estradiol for 30 minutes, respectively. The cells were marked with anti-ERalpha and measured with FACS.
The fluorescence intensity of mERalpha increased gradually with the application of estradiol. It reached the peak at the end of 30 minutes. Then it began to decrease gradually. When the cells were treated at low concentration of estradiol the fluorescence intensity of mERalpha was high, especially at 10(-10) mol/L estradiol. Then with the rise of the concentration, the fluorescence intensity decreased.
The quantity of mERalpha reached the peak at the end of 30 minutes when treated with estradiol. Then it decreased gradually. When treated with estradiol at different concentrations, its quantity could reach the peak at physiological concentration.
