Kotur-Stevuljevic Jelena, Memon Lidija, Stefanovic Aleksandra, Spasic Slavica, Spasojevic-Kalimanovska Vesna, Bogavac-Stanojevic Natasa, Kalimanovska-Ostric Dimitra, Jelić-Ivanovic Zorana, Zunic Gordana
Institute for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia.
Clin Biochem. 2007 Feb;40(3-4):181-7. doi: 10.1016/j.clinbiochem.2006.09.007. Epub 2006 Sep 30.
In addition to many traditional risk factors for coronary artery disease (CAD) development, enhanced oxidative stress and inflammation are serious conditions that may also be classified as novel risk factors. In the present study, we assessed the relationship between several parameters of oxidative stress status [malonaldehyde (MDA), superoxide anion (O(2)(-)) and plasma and erythrocyte superoxide dismutase (SOD) activities] with high sensitivity C-reactive protein (hsCRP) and fibrinogen as inflammation markers.
Oxidative stress status parameters, inflammation markers and lipid status parameters were measured in 385 subjects [188 coronary heart disease (CHD) patients with angiographically diagnosed coronary artery disease (CAD), 141 patients with occlusion >50% in at least one major coronary artery (CAD+) and 47 patients with occlusion less than 50% (CAD-), and 197 CHD-free middle-aged subjects (the control group)].
The plasma MDA concentration and the level of O(2)(-) in plasma were significantly higher in combination with significantly lower SOD activity in the CAD+ group vs. the control group. By using multiple stepwise regression analysis, fibrinogen and hsCRP showed independent correlation with MDA. Binary logistic regression analysis indicated that both MDA and O(2)(-) were significantly associated with CAD development and adjustment for inflammatory markers weakened this association in the case of MDA.
The relationship between oxidative stress parameters and inflammatory species suggest their strong mutual involvement in atherosclerosis development that leads to CAD progression.
除了许多导致冠状动脉疾病（CAD）发生的传统危险因素外，氧化应激增强和炎症也是严重情况，也可归类为新型危险因素。在本研究中，我们评估了氧化应激状态的几个参数[丙二醛（MDA）、超氧阴离子（O(2)(-)）以及血浆和红细胞超氧化物歧化酶（SOD）活性]与作为炎症标志物的高敏C反应蛋白（hsCRP）和纤维蛋白原之间的关系。
在385名受试者中测量了氧化应激状态参数、炎症标志物和血脂状态参数[188名经血管造影诊断为冠状动脉疾病（CAD）的冠心病（CHD）患者、141名至少一条主要冠状动脉闭塞>50%的患者（CAD+）、47名闭塞小于50%的患者（CAD-）以及197名无CHD的中年受试者（对照组）]。
与对照组相比，CAD+组的血浆MDA浓度和血浆O(2)(-)水平显著更高，而SOD活性显著更低。通过多元逐步回归分析，纤维蛋白原和hsCRP与MDA显示出独立相关性。二元逻辑回归分析表明，MDA和O(2)(-)均与CAD发生显著相关，并且针对炎症标志物进行调整后，MDA与CAD的关联减弱。
氧化应激参数与炎症物质之间的关系表明它们在动脉粥样硬化发展中强烈相互关联，而动脉粥样硬化发展会导致CAD进展。
