Guioli Silvana, Sekido Ryohei, Lovell-Badge Robin
Division of Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
Dev Biol. 2007 Feb 15;302(2):389-98. doi: 10.1016/j.ydbio.2006.09.046. Epub 2006 Oct 3.
In vertebrates the female reproductive tracts derive from a pair of tubular structures called Mullerian ducts, which are composed of three elements: a canalised epithelial tube, mesenchymal cells surrounding the tube and, most externally, coelomic epithelial cells. Since the first description by Johannes Peter Muller in 1830, the origin of the cells making up the Mullerian duct has remained controversial. We report the results from lineage-tracing experiments in chicken and mouse embryos aimed to provide information of the dynamics of Mullerian duct formation. We show that all Mullerian duct components derive from the coelomic epithelium in both species. Our data support a model of a Mullerian epithelial tube derived from an epithelial anlage at the mesonephros anterior end, which then segregates from the epithelium and extends caudal of its own accord, via a process involving rapid cell proliferation. This tube is surrounded by mesenchymal cells derived from local delamination of coelomic epithelium. We exclude any significant influx of cells from the Wolffian duct and also the view of a tube forming by coelomic epithelium invagination along the mesonephros. Our data provide clues of the underlying mechanism of tubulogenesis relevant to both normal and abnormal development of the female reproductive tract.
在脊椎动物中，雌性生殖道起源于一对称为缪勒管的管状结构，它由三个部分组成：一个形成管道的上皮管、围绕该管的间充质细胞以及最外层的体腔上皮细胞。自1830年约翰内斯·彼得·米勒首次描述以来，构成缪勒管的细胞来源一直存在争议。我们报告了在鸡和小鼠胚胎中进行的谱系追踪实验结果，旨在提供有关缪勒管形成动态的信息。我们表明，在这两个物种中，缪勒管的所有组成部分均源自体腔上皮。我们的数据支持一种模型，即缪勒上皮管起源于中肾前端的上皮原基，然后通过一个涉及快速细胞增殖的过程从上皮中分离出来，并自行向尾端延伸。该管被源自体腔上皮局部脱层的间充质细胞所包围。我们排除了来自沃尔夫管的任何大量细胞流入，以及体腔上皮沿中肾内陷形成管道的观点。我们的数据为与雌性生殖道正常和异常发育相关的管形成潜在机制提供了线索。
