Carpenter M B, Jayaraman A
Department of Anatomy, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
J Hirnforsch. 1990;31(5):653-68.
Retrograde and anterograde transport of wheat germ agglutinin-horseradish peroxidase (WGA-HRP) was studied in 7 squirrel monkeys with discrete injections of the subthalamic nucleus (STN). Injections labeled: (1) the lateral two-thirds of the nucleus (63% and 47%), (2) ventrolateral parts caudally (20%), (3) dorsomedial parts caudally (18%), (4) rostromedial parts (21%), (5) the medial third (38%) and (6) the lateral pole of the nucleus (9%). Afferents to the lateral two-thirds of the STN originated from two parallel cellular arrays in dorsal parts of the middle third of the lateral pallidal segment (LPS) and a single array in the rostral third of the LPS. Medial regions of the STN received input from cells in the rostral LPS. Small numbers of cells were retrogradely labeled in the centromedian-parafascicular (CM-PF) and the pedunculopontine (PPN) nuclei. No cells were labeled in the frontal cortex, the striatum, the substantia innominata (SI), the substantia nigra (SN) or the dorsal nucleus of the raphe. Virtually all pallidal neurons, including identified pallidosubthalamic neurons, were immunoreactive (IR) for gamma-aminobutyric acid (GABA). Pallidosubthalamic neurons were most numerous in regions of the LPS with the lowest density of leucine enkephalin-IR fibers. Substance P-IR fibers, found mainly in the medial pallidal segment, bore no relationship to pallidal afferents to the STN. Choline acetyltransferase-IR cells in the SI and the PPN were not retrogradely labeled with WGA-HRP granules. Anterograde transport in fibers and terminal fields surrounded retrogradely labeled cells in the LPS, suggesting a reciprocal relationship. The caudal third of the LPS and ventral region of the middle third of this nucleus, appeared to project few fibers to, or to receive few fibers from, the STN. A small number of STN efferents entered the medial border of the putamen, but no terminal fields were identified. STN projections to the pars reticulata of the SN appeared to represent about 10% of the projection to the LPS. No STN efferents were identified in the frontal cortex, the SI or the PPN. The hypothesis that STN afferents from the frontal cortex and CM-PF may represent collaterals of projections to other loci is discussed.
在7只松鼠猴中，通过向丘脑底核（STN）进行离散注射，研究了小麦胚凝集素 - 辣根过氧化物酶（WGA - HRP）的逆行和顺行运输。注射标记了：（1）核的外侧三分之二（63%和47%），（2）尾侧腹外侧部分（20%），（3）尾侧背内侧部分（18%），（4）嘴侧内侧部分（21%），（5）内侧三分之一（38%）和（6）核的外侧极（9%）。STN外侧三分之二的传入纤维起源于外侧苍白球段（LPS）中三分之一背侧部分的两个平行细胞阵列以及LPS嘴侧三分之一中的单个阵列。STN的内侧区域接受来自嘴侧LPS中细胞的输入。在中央中 - 束旁核（CM - PF）和脚桥核（PPN）中有少量细胞被逆行标记。在额叶皮质、纹状体、无名质（SI）、黑质（SN）或中缝背核中没有细胞被标记。几乎所有苍白球神经元，包括已鉴定的苍白球 - 丘脑底核神经元，对γ - 氨基丁酸（GABA）呈免疫反应（IR）。苍白球 - 丘脑底核神经元在LPS中亮氨酸脑啡肽 - IR纤维密度最低的区域数量最多。主要在苍白球内侧段发现的P物质 - IR纤维与苍白球到STN的传入纤维无关。SI和PPN中的胆碱乙酰转移酶 - IR细胞没有被WGA - HRP颗粒逆行标记。纤维和顺行运输的终末场围绕着LPS中逆行标记的细胞，表明存在相互关系。LPS的尾侧三分之一和该核中间三分之一的腹侧区域似乎向STN投射的纤维很少，或从STN接收的纤维很少。少量STN传出纤维进入壳核的内侧边界，但未发现终末场。STN向SN网状部的投射似乎占向LPS投射的约10%。在额叶皮质、SI或PPN中未发现STN传出纤维。讨论了额叶皮质和CM - PF的STN传入纤维可能代表投射到其他位点的侧支的假说。
