Carpenter M B, Periera A B, Guha N
Department of Anatomy, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
J Hirnforsch. 1992;33(2):151-67.
Attempts were made to co-define afferents of the oculomotor nuclear complex (OMC) and their putative neurotransmitters in the squirrel monkey. Wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) and wheat germ agglutinin conjugated to enzymatically inactive HRP and coupled to colloidal gold (WGAapoHRP-AU) were used as retrograde tracers in combination with immunocytochemical methods. Primarily unilateral injections were made into portions of the OMC. Stabilized tetramethylbenzidine (TMB) and silver enhanced sections were immunoreacted with antisera for choline acetyltransferase (ChAT), glutamate (GLU), aspartate (ASP), aminobutyric acid (GABA), serotonin (5-HT) and cholecystokinin (CCK). Moderate numbers of ChAT-IR neurons in caudal regions of the medial vestibular nuclei (MVN) projected to the OMC. Tracer labeled ChAT-IR cells in the MVN projected ipsilaterally to the ventral nucleus (medial rectus subdivision) of the OMC and bilaterally with contralateral dominance to other OMC subdivisions. Cholinergic neurons in the dorsal paragigantocellular reticular nucleus (DPG) projected bilaterally to each half of the OMC. Cells of the DPG, considered to contain inhibitory burst neurons impinging upon the contralateral abducens nucleus, were shown to project to virtually all subdivision of the OMC. Abducens motor neurons were ChAT-IR, but abducens internuclear neurons were not. Cells in caudal parts of the nucleus prepositus (NPP) projecting to the ipsilateral ventral nucleus of the OMC were not ChAT-positive; ChAT-IR cells in rostral NPP did not project to the OMC. Unilateral OMC injections labeled cells ipsilaterally in the RiMLF, contralaterally in the pretectal olivary nucleus, the interstitial nucleus of Cajal and the infracerebellar nucleus and bilaterally in the superior vestibular nucleus, none of which were ChAT-IR. A small number of cells in the locus ceruleus projected ipsilaterally to the OMC. Although large numbers of vestibular neurons were GLU-IR and ASP-IR, only a few tracer labeled ASP-IR neurons in the contralateral MVN projected to the OMC. No other GLU- or ASP-positive neurons were immunoreactive for GABA, 5-HT or CCK, but cells of the lateral vestibular nucleus were surrounded by CCK-IR fibers and terminals.
人们尝试共同确定松鼠猴动眼神经核复合体（OMC）的传入神经及其假定的神经递质。将与辣根过氧化物酶结合的小麦胚凝集素（WGA-HRP）以及与无酶活性的HRP结合并偶联胶体金的小麦胚凝集素（WGAapoHRP-AU）用作逆行示踪剂，并结合免疫细胞化学方法。主要进行单侧注射到OMC的部分区域。用胆碱乙酰转移酶（ChAT）、谷氨酸（GLU）、天冬氨酸（ASP）、氨基丁酸（GABA）、5-羟色胺（5-HT）和胆囊收缩素（CCK）的抗血清对稳定化的四甲基联苯胺（TMB）和银增强切片进行免疫反应。内侧前庭核（MVN）尾侧区域中数量适中的ChAT免疫反应性（ChAT-IR）神经元投射到OMC。MVN中示踪剂标记的ChAT-IR细胞同侧投射到OMC的腹侧核（内直肌亚核），并双侧投射到其他OMC亚核，以对侧为主。背侧巨细胞旁网状核（DPG）中的胆碱能神经元双侧投射到OMC的每一半。DPG的细胞被认为包含影响对侧展神经核的抑制性爆发神经元，已显示其投射到OMC的几乎所有亚核。展神经运动神经元是ChAT-IR，但展神经核间神经元不是。前庭前核（NPP）尾侧部分投射到同侧OMC腹侧核的细胞不是ChAT阳性；NPP头侧的ChAT-IR细胞不投射到OMC。单侧OMC注射标记了同侧的中脑导水管周围灰质背外侧部（RiMLF）、对侧的顶盖橄榄核、 Cajal间质核和小脑下核以及双侧的前庭上核中的细胞，这些细胞均不是ChAT-IR。蓝斑中的少量细胞同侧投射到OMC。尽管大量前庭神经元是GLU-IR和ASP-IR，但对侧MVN中只有少数示踪剂标记ASP-IR神经元投射到OMC。没有其他GLU或ASP阳性神经元对GABA、5-HT或CCK呈免疫反应，但外侧前庭核的细胞被CCK-IR纤维和终末包围。
