Medintz Igor L
Center for Bio/Molecular Science and Engineering, Code 6900 U.S. Naval Research Laboratory, 4555 Overlook Ave, SW Washington, DC 20375, USA.
Trends Biotechnol. 2006 Dec;24(12):539-42. doi: 10.1016/j.tibtech.2006.10.008. Epub 2006 Oct 30.
Intracellular sensors for nutrients such as sugars, metabolic precursors and signaling ligands such as amino acids will help to elucidate the complex roles of these small molecules in biology. In this update, several recently developed sensors, originating from the Frommer laboratory, which combine bacterial periplasmic-binding protein-based specificity for ligand targeting with fluorescent protein-derived resonance energy transfer for signal transduction are reviewed. The insight gained from designing these sensors, along with the preliminary results gathered from their first application, serve to illustrate the impact that they can have on improving our fundamental understanding of biology.
用于检测糖等营养物质、代谢前体以及氨基酸等信号配体的细胞内传感器，将有助于阐明这些小分子在生物学中的复杂作用。在本次更新中，我们将综述几种最近开发的传感器，这些传感器源自弗罗默实验室，它们将基于细菌周质结合蛋白的配体靶向特异性与荧光蛋白衍生的共振能量转移用于信号转导相结合。从设计这些传感器中获得的见解，以及从它们的首次应用中收集到的初步结果，都有助于说明它们对增进我们对生物学的基本理解可能产生的影响。
