Uriel Shiri, Brey Eric M, Greisler Howard P
Pritzker Institute of Medical Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA.
Am J Surg. 2006 Nov;192(5):604-9. doi: 10.1016/j.amjsurg.2006.08.012.
Therapeutic neovascularization using high growth factor concentrations may lead to transient vessel formation and abnormal microvascular structure. The goal of this study was to quantify temporal and concentration effects of fibroblast growth factor-1 (FGF-1) on the persistence and morphology of microvascular networks.
Endothelial cells were incubated in suspension culture forming aggregates that were embedded in fibrin glue (FG) and stimulated with varying concentrations of FGF-1 with of heparin. Capillary networks formed were quantified for 21 days.
High FGF-1 concentrations resulted in rapid and intense sprout formation, with excessive branching. At later times, these vessels regressed, with cellular debris in former vessel locations. At later times, the 1-ng/mL group surpassed the high concentration groups with continuous sprout growth and complete FG vascularization by 23 days.
Sustained low levels of FGF-1 maintained a persistent microvascular network response, whereas higher levels resulted in abnormal phenotype followed by vessel regression.
使用高浓度生长因子进行治疗性血管生成可能会导致短暂的血管形成和异常的微血管结构。本研究的目的是量化成纤维细胞生长因子-1（FGF-1）对微血管网络持久性和形态的时间和浓度效应。
将内皮细胞在悬浮培养中孵育形成聚集体，将其包埋在纤维蛋白胶（FG）中，并用不同浓度的FGF-1和肝素进行刺激。对形成的毛细血管网络进行21天的量化。
高浓度的FGF-1导致快速且强烈的芽形成，并伴有过度分支。在后期，这些血管退化，先前血管位置出现细胞碎片。在后期，1 ng/mL组在23天时超过了高浓度组，持续有芽生长并实现了FG完全血管化。
持续低水平的FGF-1维持了持久的微血管网络反应，而较高水平则导致异常表型，随后血管退化。
