Kutubuddin M, Kolaskar A S, Galande S, Gore M M, Ghosh S N, Banerjee K
National Institute of Virology, Pune, India.
Mol Immunol. 1991 Jan-Feb;28(1-2):149-54. doi: 10.1016/0161-5890(91)90098-5.
Helper T (Th) cell antigenic sites were predicted from the primary amino acid sequence (approximately 500 in length) of the envelope (E) glycoprotein (gp) of Japanese encephalitis (JE), West Nile (WN) and Dengue (DEN) I-IV flaviviruses. Prediction of Th epitopes was done by analyzing the occurrence of amphipathic segments, Rothbard-Taylor tetra/pentamer motifs and presence of alpha helix-preferring amino acids. The simultaneous occurrence of all these parameters in segments of E gp were used as criteria for prediction as Th epitopes. Only one cross reactive epitope was predicted in the C-terminal region of the E gp predicted segments of all flaviviruses analyzed. This region is one of the longest amphipathic stretch (approximately from 420 to 455) and also has a fairly large amphipathic score. Based on the predicted findings three selected peptides were synthesized and analyzed for their ability to induce in vitro T cell proliferative response in different inbred strains of mice (Balb/c, C57BL6, C3H/HeJ). Synthetic peptide I and II prepared from C-terminal region gave a cross reactive response to JE, WN and Den-II in Balb/c and C3H/HeJ mice. Synthetic peptide III prepared from N-terminal region gave a proliferative response to DEN-II in Balb/c strain only, indicating differential antigen presentation.
从日本脑炎（JE）、西尼罗河病毒（WN）以及登革热病毒（DEN）I - IV型黄病毒包膜（E）糖蛋白（gp）的一级氨基酸序列（长度约为500个氨基酸）预测辅助性T（Th）细胞抗原位点。通过分析两亲性片段的出现情况、Rothbard - Taylor四聚体/五聚体基序以及偏好α螺旋的氨基酸的存在情况来预测Th表位。E gp片段中所有这些参数同时出现被用作预测Th表位的标准。在所分析的所有黄病毒的E gp预测片段的C端区域仅预测到一个交叉反应性表位。该区域是最长的两亲性延伸区之一（大约从420到455），并且具有相当高的两亲性得分。基于预测结果，合成了三种选定的肽，并分析了它们在不同近交系小鼠（Balb/c、C57BL6、C3H/HeJ）中诱导体外T细胞增殖反应的能力。从C端区域制备的合成肽I和II在Balb/c和C3H/HeJ小鼠中对JE、WN和登革热病毒II型产生交叉反应。仅从N端区域制备的合成肽III仅在Balb/c品系中对登革热病毒II型产生增殖反应，表明抗原呈递存在差异。
