Wani S A, Samanta I, Pandit F, Buchoo B A, Peer F, Bhat M A
Division of Microbiology and Immunology, S. K. University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar-190006, India.
Vet Rec. 2006 Oct 28;159(18):587-90. doi: 10.1136/vr.159.18.587.
The aims of this investigation were to determine the prevalence of ovine herpesvirus type 2 (OvHV-2) (the causative agent of malignant catarrhal fever) infection in cattle, the carrier status of sheep and goats, and to define the pattern of acquisition of OvHV-2 in lambs under natural flock conditions in Kashmir, India. None of the buffy coat samples from 21 lambs contained OvHV-2 DNA sequences up to 28 days after birth, only one lamb had sequences of OvHV-2 DNA as early as 29 days after birth, and they were detected in the other 20 lambs when they were between 43 and 94 days of age. Sequences of OvHV-2 DNA were detected in buffy coat samples from 28 (85 per cent) of 33 adult sheep and in 16 (61 per cent) of 26 samples from adult goats by hemi-nested PCR. Seventeen (31 per cent) of 55 cattle with malignant catarrhal fever-like clinical signs had sequences of OvHV-2 DNA in their blood, and nine of the 17 died, all of them during the months of April to November, between November 2002 and March 2004. No clinical cases of sheep-associated malignant catarrhal fever was recorded during the months of December to March. The overall prevalence of OvHV-2 infection in the cattle in the region was estimated to be less than 1 per cent.
本调查的目的是确定牛群中2型绵羊疱疹病毒（OvHV-2，恶性卡他热的病原体）感染的流行情况、绵羊和山羊的携带状态，并确定印度克什米尔自然羊群条件下羔羊感染OvHV-2的模式。21只羔羊的血沉棕黄层样本在出生后28天内均未检测到OvHV-2 DNA序列，只有1只羔羊在出生后29天最早检测到OvHV-2 DNA序列，其他20只羔羊在43至94日龄时检测到该序列。通过半巢式PCR在33只成年绵羊的28份（85%）血沉棕黄层样本以及26份成年山羊样本的16份（61%）中检测到OvHV-2 DNA序列。55头有恶性卡他热样临床症状的牛中，17头（31%）血液中有OvHV-2 DNA序列，其中9头死亡，均在2002年11月至2004年3月期间的4月至11月。12月至3月期间未记录到与绵羊相关的恶性卡他热临床病例。该地区牛群中OvHV-2感染的总体流行率估计低于1%。
