Yurist Sophie, Dahan Idit, Eichler Jerry
Department of Life Sciences, Ben Gurion University, P.O. Box 653, Beersheva 84105, Israel.
J Bacteriol. 2007 Jan;189(1):276-9. doi: 10.1128/JB.01410-06. Epub 2006 Oct 27.
In vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted. Although normally transcribed at a level comparable to that of the essential SRP54 gene, SRP19 deletion had no effect on cell growth, membrane protein insertion, protein secretion, or ribosome levels. The absence of SRP19 did, however, increase membrane bacterioruberin levels.
在体外,古菌的信号识别颗粒54(SRP54)在没有信号识别颗粒19(SRP19)的情况下能结合信号识别颗粒RNA,这表明后者在古菌中是可有可无的。因此,删除了嗜盐栖热袍菌的SRP19基因。尽管SRP19基因的正常转录水平与必需的SRP54基因相当,但删除SRP19对细胞生长、膜蛋白插入、蛋白质分泌或核糖体水平没有影响。然而,SRP19的缺失确实增加了膜细菌红素的水平。