Zubov A N, Pisareva L N
Tsitologiia. 1990;32(10):1019-22.
Blood serum gamma-globulin and albumin were tested for their effect on the sodium currents of voltage clamped internally perfused serum deprived neuroblastoma cells. Albumin in the concentration corresponding to its content in 5% blood serum (22 microM) produced an increase in sodium channel currents and shifts in activation and inactivation curves along the voltage axis towards more negative or positive potentials, respectively. These results both qualitatively and quantitatively reproduce the effects of 5% blood serum observed previously (Zubov, Sal'nikov, 1984, 1986). The addition of gamma-globulin failed to change the parameters registered. The data obtained led us to an assumption of the role of albumin as an active substance responsible for the effect of blood serum on the potential-dependent sodium-transporting system of neuroblastoma cell membrane.
检测了血清γ-球蛋白和白蛋白对电压钳制下内部灌注血清剥夺神经母细胞瘤细胞钠电流的影响。浓度相当于其在5%血清中的含量（22微摩尔）的白蛋白使钠通道电流增加，激活曲线和失活曲线分别沿电压轴朝着更负或更正的电位移动。这些结果在定性和定量上都重现了先前观察到的5%血清的作用（祖博夫、萨尔尼科夫，1984年、1986年）。添加γ-球蛋白未能改变所记录的参数。所获得的数据使我们推测白蛋白作为一种活性物质，负责血清对神经母细胞瘤细胞膜电位依赖性钠转运系统的作用。
