Imberty Anne, Wimmerová Michaela, Koca Jaroslav, Breton Christelle
Centre de Recherches sur les Macromolécules Végétales, CNRS, Grenoble, France.
Methods Mol Biol. 2006;347:145-56. doi: 10.1385/1-59745-167-3:145.
Glycosyltransferases, the enzymes that build oligosaccharides and glycoconjugates, have received much interest in recent years owing to their biological functions and their potential uses in biotechnology. The analysis of the wealth of sequences that are now available in databases allowed the classification in different families characterized by conserved peptide motifs. Nevertheless, only a limited number of crystal structures is available and molecular modeling appears to be an inescapable tool for rationalization of binding data, engineering of enzyme properties, and design of inhibitors that would be of interest as therapeutic compounds. Because of sequence diversity and limited experimental data, molecular modeling of these enzymes is not straightforward and utilizes the most recent tools, such as fold recognition programs.
糖基转移酶是构建寡糖和糖缀合物的酶，近年来因其生物学功能以及在生物技术中的潜在用途而备受关注。对数据库中现有大量序列的分析使得基于保守肽基序的不同家族得以分类。然而，仅有数量有限的晶体结构可供使用，分子建模似乎成为解释结合数据、改造酶特性以及设计作为治疗化合物的抑制剂等方面不可或缺的工具。由于序列多样性和实验数据有限，这些酶的分子建模并非易事，需要利用诸如折叠识别程序等最新工具。
