Marolda Cristina L, Lahiry Piya, Vinés Enrique, Saldías Soledad, Valvano Miguel A
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada.
Methods Mol Biol. 2006;347:237-52. doi: 10.1385/1-59745-167-3:237.
Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.
从细菌全细胞裂解物或膜制剂中快速简单分析脂多糖（LPS）的方法有助于推进我们对LPS生物合成遗传学的认识。LPS是革兰氏阴性菌外膜的主要成分，具有复杂的合成和组装机制，需要许多基因和基因产物的协同参与。本章介绍了我们实验室常规用于表征大肠杆菌和其他细菌中LPS的一系列方法。
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