Minka S, Bruneteau M
Laboratoire d'écologie microbienne du sol, Unité mixte de recherche, Centre national de la recherche scientifique, Université Claude Bernard, Villeurbanne, France.
Can J Microbiol. 1998 May;44(5):477-81.
The lipopolysaccharides LPS I and LPS II, isolated from the hypovirulent EV40 strain of Yersinia pestis, are composed only of type R lipopolysaccharides. This type consists of two forms a and b, depending on their solubility pattern in a solvent mixture containing varying proportions of chloroform, methanol, hexane, and hydrochloric acid. LPS I consists of one subtype, RIb, while LPS II consists of two subtypes, RIIa and RIIb. Analysis by gel electrophoresis shows that the mass of these lipopolysaccharide forms are in the vicinity of 2000-3000 Da. The RIb and RIIb subtypes, which are found in the majority of lipopolysaccharide I and II fractions, are composed of ketoses and amines that are similar to those occurring in LPS I and LPS II. In contrast, the two subtypes RIIa and RIIb are different both in terms of the composition of lipid A and the extent of its substitution. Certain fractions of RIIa contain only lipid A and 3-deoxy-D-manno-octulosonic acid (KDO), while other fractions of RIIb possess a lipid A, which is not substituted by arabinose. The whole set of these R-type lipopolysaccharide forms are excellent models for the study of the role of the primary structure of the polysaccharide region, and for the effect of lipid A substitution on the biological activity of bacterial lipopolysaccharides.
从低毒力鼠疫耶尔森氏菌EV40菌株中分离出的脂多糖LPS I和LPS II仅由R型脂多糖组成。根据其在含有不同比例氯仿、甲醇、己烷和盐酸的溶剂混合物中的溶解模式，这种类型有a和b两种形式。LPS I由一种亚型RIb组成，而LPS II由两种亚型RIIa和RIIb组成。凝胶电泳分析表明，这些脂多糖形式的质量在2000 - 3000 Da左右。在大多数脂多糖I和II组分中发现的RIb和RIIb亚型由与LPS I和LPS II中存在的酮糖和胺类似的成分组成。相比之下，RIIa和RIIb这两种亚型在脂质A的组成及其取代程度方面都有所不同。RIIa的某些组分仅含有脂质A和3 - 脱氧 - D - 甘露糖辛酸（KDO），而RIIb的其他组分拥有一种未被阿拉伯糖取代的脂质A。这些R型脂多糖形式的整个集合是研究多糖区域一级结构的作用以及脂质A取代对细菌脂多糖生物活性影响的优秀模型。
