体外O抗原连接酶测定。
In vitro O-antigen ligase assay.
作者信息
Ruan Xiang, Valvano Miguel A
机构信息
Department of Microbiology and Immunology, Centre for Human Immunology, University of Western Ontario, London, ON, Canada.
出版信息
Methods Mol Biol. 2013;1022:185-97. doi: 10.1007/978-1-62703-465-4_15.
PMID:23765663
Abstract
WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.
摘要
WaaL是一种膜酶，它催化十一异戊二烯二磷酸（Und-PP）-O抗原近端的糖与脂多糖核心寡糖（OS）的末端糖之间的糖苷键形成。这是脂多糖合成中的关键步骤。我们在此描述一种利用天然底物在体外进行连接反应的测定方法。
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