• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates.

作者信息

Vilardo Mônica de Castro Britto, Thomé Jacqueline Darc da Silva, Esteves Wagner Thadeu Cardoso, Filgueiras Ana Luzia Lauria, de Oliveira Selma Soares

机构信息

Laboratório de Zoonoses Bacterianas, Departamento de Bacteriologia, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil

出版信息

Mem Inst Oswaldo Cruz. 2006 Aug;101(5):499-501. doi: 10.1590/s0074-02762006000500003.

DOI:10.1590/s0074-02762006000500003
PMID:17072452
Abstract

A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.

摘要

相似文献

1
Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates.
Mem Inst Oswaldo Cruz. 2006 Aug;101(5):499-501. doi: 10.1590/s0074-02762006000500003.
2
Identification of Campylobacter species isolates with phenotypic methods and polymerase chain reaction.用表型方法和聚合酶链反应鉴定弯曲杆菌属菌株
Srp Arh Celok Lek. 2014 Nov-Dec;142(11-12):708-12. doi: 10.2298/sarh1412708m.
3
Identification of thermophilic Campylobacter spp. by phenotypic and molecular methods.通过表型和分子方法鉴定嗜热弯曲杆菌属。
J Appl Microbiol. 2001 Mar;90(3):470-5. doi: 10.1046/j.1365-2672.2001.01267.x.
4
Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA.通过基于脂质A基因lpxA核苷酸序列开发的多重PCR对空肠弯曲菌、结肠弯曲菌、拉氏弯曲菌和乌普萨拉弯曲菌进行鉴别。
J Clin Microbiol. 2004 Dec;42(12):5549-57. doi: 10.1128/JCM.42.12.5549-5557.2004.
5
Molecular-based identification and typing of Campylobacter jejuni and C. coli.空肠弯曲菌和大肠弯曲菌基于分子的鉴定与分型
Methods Mol Biol. 2004;268:33-47. doi: 10.1385/1-59259-766-1:033.
6
Validation of a polymerase chain reaction/restriction enzyme analysis method for species identification of thermophilic campylobacters isolated from domestic and wild animals.用于从家畜和野生动物中分离的嗜热弯曲杆菌进行菌种鉴定的聚合酶链反应/限制性酶切分析方法的验证
J Appl Microbiol. 2002;92(1):47-54. doi: 10.1046/j.1365-2672.2002.01491.x.
7
Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from diarrheal patients in Japan.评价基于细胞致死扩张毒素 (cdt) 基因的种特异性多重 PCR 检测方法，用于鉴定从日本腹泻患者中分离的弯曲菌菌株。
Jpn J Infect Dis. 2011;64(1):19-27.
8
Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus.用于空肠弯曲菌、大肠弯曲菌、海鸥弯曲菌、乌普萨拉弯曲菌和胎儿弯曲菌胎儿亚种鉴定及区分的菌落多重聚合酶链反应检测
J Clin Microbiol. 2002 Dec;40(12):4744-7. doi: 10.1128/JCM.40.12.4744-4747.2002.
9
[Evaluation of genotypic and phenotypic methods for the differentiation of Campylobacter jejuni and Campylobacter coli clinical isolates from Poland. II. PFGE, ERIC-PCR, PCR-flaA-RFLP and MLST].[波兰空肠弯曲菌和结肠弯曲菌临床分离株分型及表型鉴定方法的评估。II. 脉冲场凝胶电泳、肠杆菌基因间重复一致序列聚合酶链反应、flaA基因聚合酶链反应-限制性片段长度多态性分析及多位点序列分型]
Med Dosw Mikrobiol. 2009;61(1):63-77.
10
Comparative performance of different PCR assays for the identification of Campylobacter jejuni and Campylobacter coli.用于鉴定空肠弯曲菌和结肠弯曲菌的不同聚合酶链反应检测方法的比较性能
Res Microbiol. 2008 Mar;159(2):88-93. doi: 10.1016/j.resmic.2007.11.020. Epub 2008 Jan 4.

引用本文的文献

1
Characterization of Campylobacter jejuni, Campylobacter upsaliensis, and a novel Campylobacter sp. in a captive non-human primate zoological collection.空肠弯曲菌、乌普萨拉弯曲菌及一种新型弯曲菌在圈养非人灵长类动物动物园种群中的特征分析。
J Med Primatol. 2019 Apr;48(2):114-122. doi: 10.1111/jmp.12393. Epub 2018 Dec 9.
2
Transmission OF Campylobacter coli in chicken embryos.鸡胚中弯曲杆菌属 coli 的传播。
Braz J Microbiol. 2012 Apr;43(2):535-43. doi: 10.1590/S1517-83822012000200014. Epub 2012 Jun 1.